• ENaC

    Cumulative dose-dependent block of ENaC by Amiloride, measured on the SyncroPatch 96, a predecessor model of the SyncroPatch 384PE

ENaC | Epithelial Sodium Channel | Amiloride-sensitive Sodium Channel

Family:
Epithelial sodium channels, member of the ENaC/Degenerin superfamily

Topology:
ENaC is assembled as a heterotrimer composed of three homologous subunits α or δ, β, and γ. These subunits are encoded by four genes: SCNN1A, SCNN1B, SCNN1G, and SCNN1D. As a typical member of the ENaC/Degenerin superfamily, each of the subunits consists of two transmembrane helices and an extracellular loop.

Function:
ENaC is located in the apical membrane of polarized epithelial cells in particular in the kidney (primarily in the distal tubule), the lung, and the colon facilitating Na⁺ reabsorption across the apical membranes of epithelial cells.

 

Data and Applications

ENaC - Epithelia Sodium Channel Analysis

SP96 Enac Amiloride

icon sp96   SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) data and applications:

The data present cumulative dose-dependent block of ENaC by Amiloride. Currents were obtained performing ramps -100mV/100mV every 2 s from a Vhold=-40mV. The large window shows the effect of increasing concentrations (1 µM, 10µM, 100µM) of Compound on the ENaC currents. The IC50 with Amiloride was 2.3 µM. Data were collected with a success rate of ~70 %.

 

 

 

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