• Gramicidin

    A typical Gramicidin ion channel forming activity assay on the Orbit 16.

Gramicidin

Family:
Non-ribosomally Synthesized Channels

Description:
Gramicidin is a heterogeneous mixture of three antibiotic compounds, gramicidins A, B and C, making up 80%, 6%, and 14%, respectively, all of which are obtained from the soil bacterial species Bacillus brevis and called collectively gramicidin D.

Topology:
Gramicidin is a polypeptide. The chain assembles inside of the hydrophobic interior of the cellular lipid bilayer to form a β-helix. The helix itself is not long enough to span the membrane, but it dimerizes to form the elongated channel needed to span the whole membrane.

Function:
Gramicidin's bactericidal activity is a result of increasing the permeability of the bacterial cell membrane, allowing inorganic monovalent cations (e.g. Na+) to travel through unrestricted and thereby destroying the ion gradient between the cytoplasm and the extracellular environment.

Webinars

27.01.2016 | Webinar: Instant bilayers - just add protein.

Icon Orbit   Orbit 16 and   Icon Orbit Mini   Orbit Mini

Orbits V1 flat 250pxThis webinar covers the use of the lipid bilayer platforms from Nanion: the Orbit16 and the Orbit mini for characterization of membrane proteins like ion channels, bacterial porins and biological nanopores. Both bilayer systems support high quality low noise recordings, but differ in throughput capabilities and experimental features. The Orbit16, introduced in 2012 is a device for efficient formation of 16 lipid bilayers simultaneously, allowing for parallel bilayer-reconstitution of ion channels and nanopores.

 

Data and Applications

Gramicidin - Rapid Internal Solution Exchange

p24 4 InternExchicon pap    Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:

Switching of internal solutions during gramicidin recordings from a lipid bilayer was obtained within seconds. A lipid bilayer was formed using giant unilamellar vesicles. Currents were recorded at a holding potential of +150 mV. The internal solution was switched from HCl to KCl, resulting in lower channel conductance.

Gramicidin - Ion Channel Forming Antibiotic

Icon Orbit   Orbit16 Ionera GramicidinOrbit 16 and applications:
Data were kindly provided by Ionera.

Screenshots of a recording window of a typical Gramicidin ion channel forming activity assay on the Orbit 16.
Conditions: symmetrical 0,1 HCl, +150 mV.

Gramicidin - Bilayer Recordings

p37 2 BilayRec

icon pl   Patchliner data and applications:

With suction the GUVs are attracted to the aperture. As soon as one GUV hits the glass substrate, it bursts and forms a bilayer across the aperture. Shown are single channel recordings from gramicidin which was incorprated into the bilayer after its formation. Traces were recorded in 100 mM HCl at −100 mV.

Gramicidin - Single Channel Analysis

application gramicidin 1icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:
Recordings were kindly supplied by Tohoku University, Tohoku, Japan.

Plotting the current amplitude vs. the voltage reveals conductances of 94.88 pS and 28.28 pS, which correspond to two different gramicidin derivates present in the bilayer. Traces were recorded in 100 mM HCl at the indicated potentials. Clearly two gramicidin derivates (94.88 pS and 28.28 pS) can be distinguished.

Publications

2016 - Membrane pyrophosphatases from Thermotoga maritima and Vigna radiata suggest a conserved coupling mechanism

Icon N1  SURFE²R N1 publication in Nature Communications (2016)

2015 - Automated Formation of Lipid Membrane Microarrays for Ionic Single-Molecule Sensing with Protein Nanopores

Icon Orbit  Orbit 16 publication in Small (2015)

2012 - Natural and artificial ion channels for biosensing platforms

icon pap   Port-a-Patch,   icon pl   Patchliner,   icon sp96   SyncroPatch 96 ((a predecessor model of SyncroPatch 384PE) and   icon vpp   Vesicle Prep Pro publication in Analytical and Bioanalytical Chemistry (2012)

2008 - Planar patch clamp: Advances in electrophysiology

icon pap  Port-a-Patch book chapter in "Potassium Channels" (2008)

2003 - Simultaneous optical and electrical recording of single gramicidin channels

icon pap  Port-a-Patch publication in Biophysical Journal (2003)

 

 

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Nanion Technologies GmbH

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D-80339 Munich - Germany
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