KV1.5 | Shaker Related Potassium Channel Member 5

Family:
Potassium channels

Subgroups:
Shaker (KV1.1–KV1.8), Shab (KV2.1-KV2.2), Shaw (KV3.1–KV3.4), Shal (KV4.1–KV4.3), KQT like (KV7.1–KV7.5), Eag related (KV10.1-KV10.2), Erg related (KV11.1–KV11.3), Elk related (KV12.1)

Topology:
Contains six transmembrane domains (S1–S6), four single subunits form a pore, homotetramers and heterotetramers are possible.

KV1.5 Background Information

KV1.5 belongs to the delayed rectifier class. It can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well. Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation. Homotetrameric channels display rapid activation and slow inactivation. The function of KV1.5 restores the resting membrane potential of beta cells after depolarization and thereby contributes to the regulation of insulin secretion. Expressed in atrial myocytes KV1.5 forms the IKur current. It is also expressed in the human ventricle where it is possible that it contributes to the K+ current through formation of heteromultimeric Potassium channels with other KV-alpha subunits.

Gene:
KCNA5

Human Protein:
UniProt P22460

Tissue:
Cardiac and smooth muscle, beta cells, colon, aorta, stomach and pulmonary artery, neurons, kidney

Function/ Application:
Excitability, repolarization cardiac action potential, insulin secretion, pulmonary vascular function, cardiac IKur current

Pathology:
Atrial fibrillation type 7, familial atrial fibrillation, insulinoma, sudden cardiac death, prolonged PR interval, palpitations, prolonged QTc interval, autosomal dominant inheritance

Interaction:
KVβ1, KVβ2, KVβ3.1, SAP97 , Src Tyrosine Kinase, caveolin 1, caveolin 3, α-actinin-1, α-actinin-2, DLG1, DLG4, CACNA1C

Modulator:
α-linolenic acid, TEA, 4-AP, nifedipine, flecainide, bupivacaine, E-4031, XE 991 dihydrochloride

Assays:
Patch Clamp: whole cell, temperature control

Recommended Reviews:
Gutman et al. (2005) International Union of Pharmacology. LIII. Nomenclature and molecular relationships of voltage-gated potassium channels. Pharmacol Rev 57(4):473-508

Data and Applications

KV1.5 - Dose response curve of 4-AP

icon sp96   KV1.5 Data 3845PESyncroPatch 384PE data and applications:
Cells were kindly provided by Charles River.

Screenshots of the PatchControl 384 software showing a dose-response curve of 4-AP on KV1.5 stably transfected cells. Measured on the SyncroPatch 384PE using multi-hole chips (4 holes per well), the success rate of valuable data for the analysis was 100%. The IC50 value of 160 µM measured on the SyncroPatch 384PE corresponds well to literature (IC50 4-AP: 270 µM; Gutman et al., Pharmacological Reviews 57: 473-508, 2005). 

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