KV4.3 | Shal Related Potassium Channel Member 3

Family:
Potassium channels

Subgroups:
Shaker (KV1.1–KV1.8), Shab (KV2.1-KV2.2), Shaw (KV3.1–KV3.4), Shal (KV4.1–KV4.3), KQT like (KV7.1–KV7.5), Eag related (KV10.1-KV10.2), Erg related (KV11.1–KV11.3), Elk related (KV12.1)

Topology:
Contains six transmembrane domains (S1–S6), four single subunits form a pore, homotetramers and heterotetramers are possible.

KV4.3 Background Information

KV4.3, a voltage-activated A-type potassium ion channel, is prominent in the repolarization phase of the action potential and contributes to the cardiac transient outward potassium current (Ito1), the main contributing current to the repolarizing phase 1 of the cardiac action potential. Furthermore it contributes to ISA currents in neurons.

Gene:
KCND3

Human Protein:
UniProt Q9UK17-1, Q9UK17-2 (two different isoforms encoded by alternatively spliced transcript variants of the gene)

Tissue:
Heart, brain

Function/ Application:
repolarization of action potentials

Pathology:
Brugada Syndrome 9 (gain of function mutation), Spinocerebellar Ataxia 19, autosomal dominant inheritance, cerebellar atrophy, cognitive impairment, cogwheel rigidity, dysarthria

Interaction:
Homotetramer or heterotetramer with KCND1 and/or KCND2. Associates with the regulatory subunits KCNIP1, KCNIP2, KCNIP3 and KCNIP4, KCNE1, KCNE2, SCN1B, SRC, KCNAB1 and DLG1

Modulator:
4-AP, imipramine, E-4031, amitriptyline, disopyramide, XE 991 dihydrochloride

Assays:
Patch Clamp: whole cell

Recommended Reviews:
Gutman et al. (2005) International Union of Pharmacology. LIII. Nomenclature and molecular relationships of voltage-gated potassium channels. Pharmacol Rev 57(4):473-508

Data and Applications

KV4.3/KChIP2 - Dose-response curve

icon sp96   170922 KV4.3 Data SyncroPatch384PESyncroPatch 384PE data and applications:
Cells were kindly provided by Charles River.

Screenshots of the PatchControl 384 software showing KV4.3/KChIP2 current traces in response to a voltage step protocol and the corresponing current-voltage relationship plot. Using whole cell mode in combination with multi-hole chips (4 holes per well), stably transfected cells were measured on the SyncroPatch 384PE. The IC50 value of flecanaide was determined as 28.3 µM which is in accordance to literature. The success rate of valuable data for the analysis was 100%. 

Application Notes

Cardiac Ion Channels - "Simultaneous Assessment of CiPA Stipulated Ion Channels on the SyncroPatch 384PE"

icon sp96   SyncroPatch 384PE application note   logo pdf   (1.3 MB)
Cells were kindly provided by Charles River.

Publications

2015 - Novel screening techniques for ion channel targeting drugs

icon pl  Patchliner,   icon sp96   SyncroPatch 384PE and   Icon CE   CardioExcyte 96 publication in Channels (2015)

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