• nAChRα3β4

    repetitive activation by nicotine measured on the
    Patchliner


  • nAChRα3β4

    nicotine concentration response curve
    measured on the Patchliner

  • nAChRα3β4

    block by mecamylamine
    recorded on the Patchliner

AChRa3b4| nicotinic acetylcholine receptor alpha 3 beta 4

Family:
Acetylcholine Receptors

Subgroups:
The family has been devided into nicotinic acetylcholine receptors (ionotropic channels) and muscarinic acetymcholine receptors (metabotropic G-protein coupled receptors). Both subgroups are sensitive to acetylcholine.

Topology:
Nicotinic acetylcholine receptors: As typical cys-loop receptors, acetylcholine receptor subunits contain four transmembrane spanning domains TM1-TM4. Five subunits form a pore, homomeric and heteromeric combinations are possible.17 vertebrate subunits have been identified: α genes: CHRNA1 - CHRNA10; β genes: CHRNB1 . CHRNB4, as well as CHRND (delta), CHRNE (epsilon), CHRNG (gamma)
Muscarinic acetylcholine receptors: 5 receptors have been described (M1 - M5), encoded by the 5 genes CHRM1 - CHRM5. These proteins contain seven transmembrane regions and do not form homo or heteromers. 

AChRα3β4: Background Information

Gene:
CHRNA3, CHRNB4

Human Protein:
UniProt P32297, P30926

Tissue:
Brain, retina, spinal cord, peripheral neuronal tissues, including neurons of mammalian sympathetic ganglia, parasympathetic ganglia, sensory neurons such as the trigeminal ganglia and adrenal chromaffin cells

Function/ Application:
Mediates effects of acetylcholine and nicotine in specific regions of the brain, retina and spinal cord

Pathology:
Alzheimer's disease, Parkinson's, Tourette's Syndrome, depression

Accessory subunits:

Interaction:

Modulator:
DHβE, mecamylamine, α-conotoxin, hexamethonium, A-867744, NS1738

Assays:
Patch Clamp: whole cell, room temperature

Cell systems:
Stably-transfected HEK293 cell line

Recommended Reviews:
Physiol Rev 89: 73–120, 2009; doi:10.1152/physrev.00015.2008, Albuquergue et al; Nicotinic acetylcholine receptors, in: Burger’s Medicinal Chemistry, Vol II Drug Discovery and Drug Development, Chapter 11, pp 357-405. Ed. Abraham, D., J. Wiley, New York., Colquhoun et al.

Data and Applications

Acetylcholine Receptor Alpha 3 Beta 4 - Activation

a3b4

icon sp96   SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) data and applications:

Shown is a raw current response of a HEK293 cell expressing AChR (α3β4) to exposure to 300 μM nicotine. Solutions were  stacked (layered) in the pipette to achieve brief exposure times.

 

Acetylcholine Receptor Alpha 3 Beta 4 - Concentration Response Curve to Nicotine

nAChRa3b4_nic_DRC_PL

icon pl  Patchliner data and applications:

Shown are the a raw current responses of a HEK293 cell expressing AChR (α3β4) to increasing concentrations of nicotine. Solutions were  stacked (layered) in the pipette to achieve brief exposure times.

 

Application Notes

 

 

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