• OmpF/ OmpC

    Representative recording from a single OmpF trimer showing current blocks due to the translocation of antibiotic Enrofloxacin, measured on the Orbit mini

OmpF / OmpC - Outer Membrane Protein F and C

Family:
Outer membrane Channel Proteins, OMC family, β-Barrel and Porins Superfamily

Description:
OmpF and OmpC are two prokaryotic channel proteins which are located in the outer membrane and expressed amongst other bacteris by Escherichia coliSalmonella typhimurium. 

Topology and Function:
OmpF and OmpC forms pores that allow passive diffusion of small molecules across the outer membrane.  Both are involved in osmoregulation: The widely distributed two-component system in bacteria, EnvZ/OmpR, respond to changes in envoronmental osmolality by regulating the expression of OmpF and OmpC the way that at lower osmolalities OmpC expression is less than OMPF expression.

Data and Applications

OmpF - Current block

Icon Orbit Mini   Orbit Mini OmpF 1Orbit mini and applications:
Data were kindly provided by Ionera.

Representative recording from a single OmpF trimer showing current blocks due to the translocation of antibiotic Enrofloxacin, event averaged histogram.
Conditions: 150 mM KCl, 5 mM HEPES, 5 mM MES, pH 6, 10 mM enrofloxacin, +60 mV

OmpF - Bilayer Recordings

OMPFPolyamine beaicon vpp   Vesicle Prep Pro data and applications:
Data were kindly supplied by Prof. Mathias Winterhalter, Jacobs University, Bremen, Germany.

Channels were activated in the presence of spermine (0.5 mM). The downward spikes represent the closure events due to the effect of spermine. 

Application Notes

OmpF - "Lipid Bilayer recordings of OmpF reconstituted in Proteoliposomes "

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro application note:   logo pdf   (1.4 MB) 

Posters

Publications

2015 - Quantification of Fluoroquinolone Uptake through the Outer Membrane Channel OmpF of Escherichia coli

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of the American Chemical Society (2015)

2015 - Antibiotic translocation through porins studied in planar lipid bilayers using parallel platforms

Icon Orbit  Orbit 16,   icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Analyst (2015)

2010 - Permeation of antibiotics through escherichia coli OmpF and OmpC porins: screening for influx on a single-molecule level

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Biomolecular Screening (2010)

2009 - Port-a-Patch and Patchliner: High fidelity electrophysiology for secondary screening and safety pharmacology

icon pap  Port-a-Patch and   icon pl   Patchliner publication in Combinatorial Chemistry & High Throughput Screening (2009)

2008 - Rapid screening of membrane protein activity: electrophysiological analysis of OmpF reconstituted in proteoliposomes

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Lab-on-a-chip (2008)

 

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Nanion Technologies GmbH

Ganghoferstr. 70A
D-80339 Munich - Germany
info@nanion.de