• PepT1

    Representive traces of PepT1 overexpressed in CHO cells, measured on the SURFE²R N1. The PepT1 signal is evoked by glycylglycin and inhibited by addition of Lys[Z(NO2)]-Val.

PepT1 - Peptide Transporter 1 - SLC15A1

Family:
Proton-dependent oligopeptide Transporter also known as POT, PTR Peptide transport family or NRF family

Members:
Besides members in plants, yeast, archaea and both Gram-negative and Gram-positive bacteria, there are five known members of this family in humans: SLC15A1  - SLC15A5 (Peptide transporter 1 to Peptide transporter 4, the function of SLC15A5 is unknown) 

Topology:
Members exhibit twelve putative or established transmembrane α-helical spanners.

Function:
PepT1 is localized at the brush border membrane of the intestinal epithelium and mediates the uptake of di- and tripeptides from the lumen into the enterocytes. This protein plays an important role in the uptake and digestion of dietary proteins. As well, it facilitates the absorption of numerous peptidomimetic drugs.

Data and Applications

PepT1 - Pharmacology of Lys[Z(NO2)]-Val

Surfer PepT1 traces IC50Icon N1   SURFE2R N1 data and applications:

Representive traces of PepT1 overexpressed in CHO cells. PepT1 (SLC15A1) is expressed mainly in intestine and kidney, it enables uptake of oligopeptides. The PepT1 signal is evoked by glycylglycin. The transport of oligopeptides can be inhibited by addition of Lys[Z(NO2)]-Val. The IC50 of Lys[Z(NO2)]-Val was 380µM.

PepT1 - Pharmacology

Surfer N96 PepT1 Losarthan IC50 datacontrolIcon 96SE   SURFE2R N96 (a predecessor model of SURFE²R S96) data and applications:

Transport by PepT1 is inhibited by the kompetitive antagonist Losartan. When activated with 20 mM Gly-Gly, the IC50 of Losartan was 80.4 µM. Data were analysed with DataControl - the Analysis software tool for recordings on the SURFE2R N96 and SyncroPatch 96/ 384PE.

PepT1 - Evoked signal via Glycylglycin

Surfer N96 PepT1 full screenIcon 96SE   SURFE2R N96 (a predecessor model of SURFE²R 96SE) data and applications:

Graphical user interface of screening software used on the SURFE2R N96. Traces of PepT1 recorded from membrane preparations of PepT1 overexpressing HEK293 cells are presented. The PepT1 signal is evoked by 20mM glycylglycin.

 

Posters

2017 - High throughput real-time measurement of electrogenic membrane transport driven by the SLC transporters PepT1 and OcT2

Icon 96SE   SURFE²R SE96 poster, Biophysics congress 2017  logo pdf   (1.8 MB)

2017 - An emerging technique for the characterization of transport proteins: SSM-based electrophysiology

Icon N1   SURFE²R N1 and    Icon 96SE   SURFE²R SE96 poster, 19th IUPAB / 11th EBSA congress 2017  logo pdf   (3.3 MB)

2015 - "Studying Electrogenic Transporters of the SLC Family in a Parallel Way Utilizing Solid Supported Membrane Technology"

Icon 96SE   SURFE²R N96 poster (a predecessor model of the SURFE²R 96SE) Biophysics 2015   logo pdf   (0.8 MB)

2015 - "Organellar Transporters and Ion Channels - How to access their electrophysiology by using the SURFE2R technology and Planar Patch Clamp"

Icon N1   SURFE²R N1 and   icon sp96   SyncroPatch 96 (a predecessor model of the SyncroPatch 384PE) and   icon pap   Port-a-Patch poster, GRC - Organellar Channels and Transporters 2015   logo pdf   (1.6 MB)

Application Notes

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