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Collaborative Activities with Cellular Dynamics

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21.09.2017 | Cellular Dynamics International Signs Agreement with Nanion Technologies to Enhance Mutual Offerings for Researchers

MADISON, WISCONSIN, USA, September 20, 2017-- Cellular Dynamics International Signs Agreement with Nanion Technologies to Enhance Mutual Offerings for Researchers

11.10.2016 | iForum Europe

Meeting Venue: Institut du Cerveau et de la Moelle Epinière, Paris, France.

Go to the Meeting website here.

12. - 14.09.2016 | iForum 2016 USA

Meeting Venue: Sheraton Philadelphia Society Hill Hotel, USA

Go to the iForum website here.

Cardiomyocytes - "Impedance and EFP recordings of iCell Cardiomyocytes² on the CardioExcyte 96"

Icon CE   CardioExcyte 96 Application Note   logo pdf   (2.8 MB)
Cells were kindly provided by Cellular Dynamics.

2016 - Automated Patch Clamp Meets High-Throughput Screening: 384 Cells Recorded in Parallel on a Planar Patch Clamp Module

icon sp96  SyncroPatch 384PE publication in Journal of Lab Automation (2016)

2012 - Characterizing Human Ion Channels in Induced Pluripotent Stem Cell-Derived Neurons

icon pl   Patchliner publication in Journal of Biomolecular Screening (2012)

2013 - Minimized cell usage for stem cell-derived and primary cells on an automated patchclamp system

icon pl  Patchliner publication in Journal of Pharmacological and Toxicological Methods (2013)

2015 - New Easy-to-Use Hybrid System for Extracellular Potential and Impedance Recordings.

Icon CE  CardioExcyte 96 publication in Journal of Laboratory Automation (2015)

2015 - Novel screening techniques for ion channel targeting drugs

icon pl  Patchliner,   icon sp96   SyncroPatch 384PE and   Icon CE   CardioExcyte 96 publication in Channels (2015)

GABAA Receptor - Currents in iCell Neurons

GABA Bicuculline neurons

icon pl   Patchliner data and applications:
Cells were kindly provided by Cellular Dynamics.

Activation of GABAA receptor currents by 30 μM GABA and partial block of the current response by 1 μM bicuculline. Bicuculline was pre-applied for at least 30 s before co-application with GABA (30 μM). Approximately 50% of the current was blocked by 1 μM bicuculline.

Neurons (iPSC-derived) - Voltage Gated Potassium Currents

KvIV Neurons Figure3 icon pl   Patchliner data and applications:
Cells were kindly provided by Cellular Dynamics.

A Voltage-gated K+ current recorded in iCell® neurons. Current responses to a voltage step protocol. An outward K+ current can be seen in this cell.
B Corresponding IV plot from an average of 7 cells. 

Neurons (iPSC-derived) - TXX Block of Sodium Currents

TTX Neurons Figure2icon pl   Patchliner data and applications:
Cells were kindly provided by Cellular Dynamics.

A Block of Na+ current by increasing concentrations of TTX. Current was blocked by low nM concentrations of TTX indicating a TTX sensitive Na+ channel type expressed in this cell.
B Concentration response curve for TTX inhibition, IC50 = 4.9 nM (n =1).

Neurons (iPSC-derived) - Voltage-Gated Sodium Currents

NaIV Neurons Figure1icon pl   Patchliner data and applications:
Cells were kindly provided by Cellular Dynamics.

Voltage-gated Na+ current recorded in iCell® Neurons. Current responses to a voltage step protocol.
A large inward Na+ current can be seen in this cell with a K+ outward current present at positive voltages.
B Normalised IV plot from an average of 4 cells.

11.11.2011 | Nanion successfully performs automated patch clamp recordings from stem cell-derived neurons

MUNICH, GERMANY, November 11, 2011 -- Human iPS cell-derived neurons (iCell neurons) from Cellular Dynamics International were successfully used on Nanion’s 8-channel Patchliner.
To our knowledge, this is the first time stem cell derived neurons have been used on an automated patch clamp device.

Neurons - "Stem cell-derived neurons recorded on Nanion's Patchliner"

icon pl   Patchliner application note:   logo pdf   (0.4 MB)
Cells were kindly provided by Cellular Dynamics.

NaV1.5 - Analyzing iPSC-derived Cardiomyocytes

0NavLidocaineScreenicon sp96   SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) data and applications:
Cells were kindly supplied by Cellular Dynamics.

Inhibition of NaV1.5 currents in stem cell-derived cardiomyocytes (iCells) by lidocaine. Lidocaine concentrations: 6 µM, 62 µM and 620 µM. The obatined  IC50 -value was 14 µM.

 

Cardiac Action Potentials - Automated recordings from iCells

0AP Patchliner CDI

icon pl   Patchliner data and applications:
The stem cell-derived cardiomyocytes (iCell) were kindly supplied by Cellular Dynamics.

In this example both Na+ and Ca2+ mediate the action potential. When nifedipine is applied in the current clamp mode, the action potential is shortened significantly due to block of the calcium channels.

 

Cardiomyocytes - Data from different cell providers

Icon CE   CardioExcyte different ProvidersCardioExcyte data and applications:
Cells were kindly provided by Axiogenesis, Cellular Dynamics, GE Healthcare, Pluriomics, ReproCell, Takara Bio Cellartis Clonetech.

The CardioExcyte 96 allows for
• Non-invasive, label-free measurements of beating cardiomyocyte networks
• 96 recording wells in parallel with 1 ms time resolution
• Quick experiments or long-term compound effects on cardiotoxicity
• Real-time access to beating parameters
• Outstanding software for data analysis and export
• Cost effcient consumables - 96-well format

Cardiomyocytes - Effect of E4031 on the impedance and EFP signals on iCell cardiomyocytes

Icon CE   CardioExcyte E3031CardioExcyte data and applications:
Cells were kindly provided by Cellular Dynamics.

Effect of the specific hERG blocker, E4031, on the impedance and EFP signals. A Impedance signal of 12 wells in control conditions (left) and the same 12 wells after 13 mins incubation in E4031 at the concentrations indicated (right). E4031 (300 nM, 3 mins incubation) induces EAD, shown in the inset, which can lead to potentially fatal ventricular arrhythmias. B EFP signal of 12 wells in control conditions (left) and the same 12 wells after 13 mins incubation in E4031 at the concentrations indicated (right). E4031(300 nM, 30 mins incubation) also causes arrhythmic effects in the EFP mode shown in the inset.

Cardiomyocytes - Effect of the ß-adrenergic receptor agonist, Isoproterenol, on the impedance and EFP signals on iCells

Icon CE   CardioExcyte IsoproterenolCardioExcyte data and applications:
Cells were kindly provided by Cellular Dynamics.

A Impedance signal of 12 wells in control conditions (left) and the same 12 wells after 13 mins incubation in isoproterenol at the concentrations indicated. Isoproterenol acts as a heart stimulant, it increases heart rate, an effect which is highlighted in the inset where the trace in the presence of isoproterenol (300 nM; black) and the control trace (red) are shown overlaid. In the presence of even low concentrations of isoproterenol (1 nM), an increase in the beat rate is observed. B Bar graph showing beat rate before (dark blue) and after (light blue) incubation in isoproterenol at the concentrations indicated. At all concentrations, a clear increase in beat rate is observed. C EFP signal of 12 wells in control conditions (left) and the same 12 wells after 30 mins incubation in isoproterenol at the concentrations indicated (right). Isoproterenol shortens the FPD, an effect which is highlighted in the inset where the trace in the presence of isoproterenol (300 nM; black) and the control trace (red) are shown overlaid.

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