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Collaborative Activities with NCardia

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Dr. Ralf Kettenhofen - Statement about the CardioExcyte 96

Icon CE   "CardioExcyte 96 is an easy-to-use system, providing impedance-based and MEA-like cardiac safety data from a diversity of stem cell-derived cardiomyocytes, and constitutes an excellent complement to automated patch clamp-based safety screening. Concentration- and time-dependence of a compound’s potential cardiotoxicity can efficiently be obtained where the alteration of beating patterns can give a hint as to which cardiac ion channel(s) is affected, after which detailed electrophysiology investigations can be undertaken. Cardiac network responses offer a comprehensive view of a compound’s safety profile, without having to use in-vivo methods, which saves time, costs and suffering. Further on, the powerful software, used for recordings and analysis, employs comprehensive beat investigation algorithms, displaying detailed beating kinetics in real-time. Data handling and export is straightforward, easy to grasp and yet very, very powerful."

Dr. Ralf Kettenhofen, Head of Laboratory 
Ncardia, Cologne, Germany.

04. - 08.02.2017 | SLAS

Conference Venue: Walter E. Washington Convention Center, Washington DC, USA

Go to the Conference website here.

24. - 25.11.2016 | Pluriomics User Meeting

User Meeting Venue: Pluriomics BV, Biopartner building 3, Galileiweg 8, Leiden, The Netherlands

Go to the User Meeting website here.

07. - 09.09.2016 | Axiogenesis Applications Workshop 2016

Meeting Venue: Biocampus, Nattermannallee 1, Cologne

Go to the conference website here.

2016 - Automated Patch Clamp Meets High-Throughput Screening: 384 Cells Recorded in Parallel on a Planar Patch Clamp Module

icon sp96  SyncroPatch 384PE publication in Journal of Lab Automation (2016)

2016 - Next level toxicity screening: From single channel to overall cell behavior

Icon Orbit Mini   Orbit mini,   Icon CE   CardioExcyte 96 and   icon sp96   SyncroPatch 384PE poster, Meeting of the French Society of Toxinology (SFET) 2015  logo pdf   (0.9 MB)

2013 - Automated Planar Patch Clamp

icon pl   Patchliner book chapter in Ion Channels (2013)

2013 - Minimized cell usage for stem cell-derived and primary cells on an automated patchclamp system

icon pl  Patchliner publication in Journal of Pharmacological and Toxicological Methods (2013)

2015 - New Easy-to-Use Hybrid System for Extracellular Potential and Impedance Recordings.

Icon CE  CardioExcyte 96 publication in Journal of Laboratory Automation (2015)

2015 - Novel screening techniques for ion channel targeting drugs

icon pl  Patchliner,   icon sp96   SyncroPatch 384PE and   Icon CE   CardioExcyte 96 publication in Channels (2015)

Neurons - "Peri.4U and Dopa.4U stem cell-derived neurons recorded on Nanion´s Patchliner"

icon pl   Patchliner application note:   logo pdf   (0.6 MB)
Cells were kindly provided by Axiogenesis.  

Cardiomyocytes - "Impedance and EFP recordings of Cor.4U cells using Nanion’s CardioExcyte 96"

Icon CE   CardioExcyte 96 Application Note   logo pdf   (1.5 MB)
Cells were kindly provided by Axiogenesis.  

Cardiomyocytes - "Voltage and current clamp recordings of Cor.4U human iPS cell-derived cardiomyocytes on Nanion’s Patchliner"

icon pl   Patchliner application note:   logo pdf   (0.6 MB)
Cells were kindly provided by Axiogenesis.

16.09.2011 | Nanion’s automated patch clamp platforms show unparalleled results using stem cell derived cardiomyocytes

MUNICH, GERMANY, September 9, 2011 -- The Patchliner and the SyncroPatch 96 have successfully been used for compound analysis under current- and voltage clamp recording conditions using different stem cell-derived cardiomyocytes. The exceptional cell-platform-compatibility and the unique experimental possibilities offered by Nanion’s platforms open up whole new avenues for compound safety testing.

Cardiac Ion Channels - Pharmacology of Sodium Channels

p35 1 CorAticon pl   Patchliner data and applications:
Cells (Cor.AT) were kindly provided by Axiogenesis.

The pharmacology of dibucaine was investigated by the application of 0.3, 1, 3, 10 μM in the presence of 10 μM nifedipine (L-type Ca2+-current blocker). Two control additions of nifedipine (10 μM) were made before the addition of increasing concentrations of dibucaine. The IC50 value was determined as 355 ± 40 nM (n=3), which is in accordance with the literature.

Cardiac Ion Channels - Recordings from SC-Derived Cardiomyocytes

p34 4 actPot

icon pl   Patchliner data and applications:
Cells were kindly provided by Axiogenesis.

The left picture shows a typical action potential from Cor.At® cardiomyocytes. Whole cell currents recorded in the voltage clamp mode reveal cardiomyocyte-typical ion channels (right). The traces represent mERG-, L-type Ca2+- (blue, block by 50 μM nifedipine), Na+- and K+-currents (from top left to bottom right).

 

Cardiac Action Potentials - Cor.At® Cells

p14 2 CorAT

icon pap   Port-a-Patch data and applications:
Cells were kindly provided by Axiogenesis

Cor.At® cardiomyocytes are derived from mouse embryonic stem (ES) cells. Whole cell currents recorded in voltage clamp mode reveal cardiomyocyte-typical ion channels (K+, Ca2+ and Na+). Traces on the lower left show prolongation of the action potential upon application of Dofetilide (1 μM).

Download: Application Note

Cardiac Action Potentials - From SC-Derived Cardiomyocytes

AN Patchliner CorAtCardiomyocytes 1

 icon pl   Patchliner data and applications:
Cells were kindly provided by Axiogenesis.

Action potentials recorded from stem-cell derived cardiomyocyetes (Cor.At® cardiomyocytes). Action potentials are triggered by small current pulses. Effects of quinidine and lidocaine on the action potentials are shown.

Download: Application Note

  

Cardiomyocytes - "Recordings of action potentials in mouse ES cell-derived Cor.At cardiomyocytes on Nanion's Patchliner"

icon pl   Patchliner application note:   logo pdf   (0.7 MB)
Cells were kindly provided by Axiogenesis.  

Cardiomyocytes - "Action Potentials in Mouse ES Cell-Derived Cor.At Cardiomyocytes on Nanion´s Port-a-Patch"

icon pap   Port-a-Patch application note:   logo pdf   (0.6 MB)
Cells were kindly provided by Axiogenesis.

Cardiomyocytes - Data from different cell providers

Icon CE   CardioExcyte different ProvidersCardioExcyte data and applications:
Cells were kindly provided by Axiogenesis, Cellular Dynamics, GE Healthcare, Pluriomics, ReproCell, Takara Bio Cellartis Clonetech.

The CardioExcyte 96 allows for
• Non-invasive, label-free measurements of beating cardiomyocyte networks
• 96 recording wells in parallel with 1 ms time resolution
• Quick experiments or long-term compound effects on cardiotoxicity
• Real-time access to beating parameters
• Outstanding software for data analysis and export
• Cost effcient consumables - 96-well format

Cardiomyocytes - Time-dependent effect of Pentamidine on Cor.4U cardiomyocytes

Icon CE   CardioExcyte Cor4U PentamidineCardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.

Time-dependent effect of Pentamidine on Cor.4U cardiomyocytes. Pentamidine clinically causes acquired long QT syndrome, which is associated with prolonged QT intervals, tachycardias, and sudden cardiac arrest. Pentamidine delays terminal repolarization in human heart by acutely blocking cardiac inward rectifier currents. At the same time, it reduces surface expression of the cardiac potassium channel IKr/human ether à-go-go-related gene (hERG).
Insets: EFP raw traces and online analysis value FPDc in the presence of Pentamidine (10 µM) at different timepoints.

Cardiomyocytes - Tetracaine dose response curves as recorded with Cor.4U cells

Icon CE   Tetracaine Cor4UCardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.

Impedance amplitude is not changed by addition of increasing concentrations of Tetracaine (left panel), while beat rate of Cor.4U® cells is decreasing. For example, 29.6µM of Tetracaine decreased the beat rate by ~60% when compared to pre-addition values. Cumulative dose-response relationships indicate Tetracaine potency for same-well additions. Representative raw traces for impedance signals (middle panel) clearly indicate a decrease in cell monolayer beat rate with increasing concentrations of Tetracaine.
Extracellular Field Potential (EFP) spike amplitude is decreased by cumulative Tetracaine dose applications to the same monolayer of Cor.4U® hIPSC-CMs (top right), in agreement with compound mechanism of action. Representative raw traces for EFP signals (bottom graph) clearly indicate a decrease in spike amplitude.

Cardiomyocytes - Dofetilide and its concentration dependent effect on Cor.4U cells

Icon CE   Dofetilide Cor4uCardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.

Dofetilide is a class III antiarrhythmic agent, which has pro-arrhythmic potential.
It selectively blocks the rapid component of the delayed rectifier outward potassium current (IKr). Impedance and EFP recordings reveal a concentration dependent effect on e.g. amplitude and FPD as expected.

Cardiomyocytes - Nifedipine and its concentration dependent effect on Cor.4U cells

Icon CE   Nifedipine Cor4UCardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.

Nifedpinie is a dihydropyridine calcium channel blocker that primarily blocks L-type calcium channels. Impedance and EFP recordings on Cor.4U cells reveal a concentration dependent effect on impedance amplitude, beat rate and also a shortening of the FPD as expected.

Cardiomyocytes - Optogenetics meets cardiac safety

Icon CE   CardioExcyte Optogenetics 1CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.

The stimulating optical lid, CardioExcyte 96 SOL, uses LEDs for spatially uniform stimulation of cells transfected with light-gated ion channels such as Channelrhodopsin2 (ChR2).

Right graph: LPM – Light pulse per minute plotted against the recorded beat rate (average of 96 wells). ChR2 transfected Cor.4U cells are following the optical pace rate.

Cardiomyocytes - Channelrhodopsin 2 (ChR2) transfected Cor.4U cells and optical pacing

Icon CE   CardioExcyte Optogenetics 2CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.

ChR2 transfected Cor.4U cells are following the optical pace rate.

Raw data traces upon a 1 Hz, 1.5 Hz, 2 Hz., 2.5 Hz and 3 Hz stimulation rate, extracellular field potentials (top) and impedance (bottom).

 

 

 

Cardiomyocytes - Myocyte phase II study: CiPA conform analysis and arrhythmia detection

Icon CE   CardioExcyte CiPAII 1CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.

Nanion developed a CiPA conform analysis for the Myocyte phase II study. The feature comes along is included in our CiPA analysis routine. Automated arrhythmia detection is just one highlight out of many when it comes to the CardioExcyte 96 software.

23.06.2009 | Nanion and Axiogenesis present recordings

MUNICH, GERMANY, June 23, 2009 -- Nanion and Axiogenesis present parallel patch clamp recordings of action potentials from Cor.At® Cardiomyocytes Stem cell derived cardiomyocytes were analyzed using Nanion’s Patchliner® and Port-a-patch® to validate the ion channel composition, the presence of action potentials and the effects of compounds on cardiac channels.

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Nanion Technologies GmbH

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info@nanion.de