• GluR2

    Concentration dependent activation of GluR2 receptors by Na-Glutamate, measured on the Patchliner

GluR2 | GluA2 | Glutamate Receptor Ionotropic, AMPA 2

Glutamate Receptors

The Glutamate receptor family has further been divided into ionotropic and metabotropic glutamate receptors. 
The metabotropic glutamate receptor subfamily contains 8 members (mGluR1 - mGluR8)
The ionotropic glutamate receptors subfamily contains 16 members: 
AMPA receptors: GluA1 (GluR1) - GluA4 (GluR4)
Kainate receptors: GluK1 (GluR5), GluK2 (GluR6), GluK3 (GluR7) , GluK4 (KA-1), GluK5 (KA-2)
NMDA receptors: GluN1 (NR1), GluN2A (NR2A), GluN2B (NR2B), GluN2C (NR2C), GluN2D (NR2D), GluN3A (NR3A), GluN3B (NR3B)

Regulation and Function:
Ionotropic glutamate receptors are ligand-gated nonselective cation channels that allow the flow of K+, Na+ and sometimes Ca2+ in response to glutamate binding. Metabotropic glutamate receptors belong to the subfamily C of G protein-coupled receptors. Glutamate receptors are responsible for the glutamate-mediated postsynaptic excitation of neural cells, and are important for neural communication, memory formation, learning, and regulation.

Data and Applications

AMPA Receptor (GluR2) - Fast Activation

GluR2 CRC current time course

icon pl   Patchliner data and applications:
Cells were kindly provided by University of Sussex.

Shown is concentration dependent activation of GluR2 receptors (known as AMPA receptors) by 10, 30, 100 μM and 300 µM Na-Glutamate from a GluR2 expressing HEK293 cell. The rising phase is enlarged in the right graph.

AMPA Receptor (GluR2) - Current Traces

GluA2 repetitive CRCicon sp96   SyncroPatch 384PE data and applications:
Cells were kindly provided by University of Sussex.

Using a stacked solutions approach and a fast pipetting speed shortens the solution exchange rate and minimizes the ligand exposure time. This procedure allows for reproducible recordings of fast desensitizing ligand-gated receptors such as glutamate receptors. Here, repetitive activation of GluA2 receptors is shown. Receptors were activated with 100 µM Na-Glutamate for 3 times resulting in inward currents of similar peak amplitudes (A and B). The current onset time was approximately 10 ms (D). Panel C displays an example of a cumulative concentration response curve for Na-Glutamate (in mM: 0.1, 0.3 and 1).


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