• SURFE2R 96SE

    Fully automated data recording and analysis. 10,000 data points per day!
  • SURFE2R 96SE

    First high throughput instrument on the market for SSM-based electrophysiology
  • SURFE2R 96SE

    Finally high throughput label-free functional assays for transporters available
  • SURFE2R 96SE

    High signal amplification compared to patch-clamp: transport & binding assays
  • SURFE2R 96SE

    Turn-key system for efficient transporter protein analysis

SURFE²R 96SE - High Throughput Transporter Research

Just like the SURFE2R N1, the SURFE2R 96SE employs SSM-based electrophysiology to resolve small currents of transporters and pumps, but elevates the throughput to the next level by parallelization of 96 measurements. The transporter samples are added to gold coated sensors in a standard 96 well-plate format. To further reduce the user's workload, all sensor coating steps can be done by the SURFE2R 96SE itself. Any substrate can be added to activate the transporter. The resulting transport or binding currents will be detected and analyzed accordingly. The whole process, from sample preparation and measurement to data analysis, is fully automated.

Features of the SURFE2R 96SE

• Recording of 96 wells in parallel using a 96-channel low-noise amplifier
• 10,000 data points per day
• Integration into the small footprint pipetting platform CyBio® Felix
• Automatization of experimental workflows including sample preparation
• Automated data analysis and export, e.g. determination of EC50
• 8 positions for different solutions (1 well to 96 well)

Features of SSM-based electrophysiology

• > 100 targets validated, 100 peer reviewed papers using SSM-based electrophysiology
• Transporters, pumps and ligand gated channels
• Measure even electroneutral exchangers and sugar binding
• Use purified membranes and vesicles from cells or proteoliposomes
• Label-free electrical measurements
• Requires only 0.1 – 1 µg protein per sensor. This is sufficient for up to 100 experiments
• Real-time data with high time resolution of 5 ms, not single point read-out
• High signal amplification compared to patch-clamp
• Fast binding kinetics can be resolved
• EC50, IC50, rate constants, comparison of transporter variants,...

The technology employs solid-supported membrane- (SSM-) based electrophysiology which was established in the late 1990s. Details about SSM-based electrophysiology are presented in the Technology section. The SURFE2R 96SE is preferred over the SURFE2R N1 when a high throughput environment is needed. This is the case, e.g. for compound or transporter screening projects. The SURFE2R 96SE is also suitable to increase throughput for basic research. Data generation is approximately 100 times faster compared to the SURFE2R N1.

For detailed information:

SURFE2R 96SE Device & Software

The SURFE2R 96SE device


The SURFE2R (surface electrogenic event reader) technology is the only available commercial solution for SSM-based electrophysiology on the market. Nanion Technologies developed the SURFE2R N1 as an easy-to-learn all-in-one device for universities and basic research and the high-throughput device SURFE2R 96SE.

The SURFE2R 96SE is integrated into the CyBio® Felix liquid handling platform. Nanion Technologies provides the electrophysiological hardware and the computer running a software package for data recording and analysis. The device can not only be used for electrophysiological measurements, but also for sample preparation and pipetting purposes. The device can measure 10,000 data points a day in a fully automated manner. In contrast to the SURFE2R N1, the SURFE2R 96SE also comes with a dedicated data analysis software which allows for automated analysis of the massive amount of data you generate each day.

The SURFE2R 96SE uses sensors in a standard 96-well plate format. Membranes containing the protein of interest are adsorbed onto these sensors. The measurement is initiated by a substrate concentration jump. The solution exchange is controlled by the liquid handling platform CyBio® Felix. The pipetting head contains 96 pipettes which load solutions from solution positions and inject them to the sensor plate. All 96 sensors of the sensor plate are measured simultaneously, but different solutions or transporters can be used for each measurement.

The sensor plate is enclosed by a Faraday cage and connected to a 96-channel amplifier as well as 96 gold pins which work as reference electrodes. The CyBio® Felix holds up to 8 well plates for 8 to 8 x 96 different solutions which allows up to 7 sequential measurements using different conditions in each automated workflow. The CyBio® pipettor allows the measurement of fast kinetics with low solution consumption, showing a time resolution of down to 5 ms by using only 50 µl of compound solution per measurement.
Software Package


Our software department developed a software package exclusively for the SURFE2R 96SE.

• The SurfControl works as the recording software and displays the data in real-time
• DataControl works as the analysis software and can be configured to automate your data analysis
• In addition, the CyBio Composer plus pre-installed workflows operate the liquid handling platform and
communicates with the recording software

Consumables

SURFE²R 96SE Sensor Plates

SURFE2R 96SE Sensor Plate

The SURFE²R 96SE sensor plate is a proprietary innovative product by Nanion Technologies, developed for the SURFE²R 96SE. It is produced by an external partner, quality-assured in-house at Nanion headquarters and shipped from Munich to our international customers.


Material
The core structure of SURFE²R 96SE sensor plates are gold-coated sensors with 3 mm diameter. This structure is incorporated into a 96 well plate for easy handling. The sensor plate is enclosed by a Faraday cage and connected to a 96-channel amplifier during the experiment.
Available plate type
  • "SURFE²R 96SE Sensor Plate": 96-well recording plate for the SURFE²R 96SE (Order # 182001)

Validated targets: SSM-based Electrophysiology



Testimonials & Case Studies

Dr. Thomas Seeger & Karin V. Niessen - Statement about the SURFE²R Technology

Icon N1   “The SURFE²R makes extremely challenging electrophysiological targets accessible to robust routine analyses. For example, we have developed an assay for investigating nicotinic acetylcholine receptor ion channels, using membranes from the Pacific electric ray, Torpedo California. In this way, we overcome many of the well-known difficulties associated with this ion channel, which allows us to efficiently obtain information regarding compound pharmacology. Obtained pharmacology values match those of patch clamp recordings exceptionally well. We find that the SURFE²R is an excellent platform for characterization of electrogenic processes in isolated membranes.”

Karin V. Niessen, Dr. Thomas Seeger
Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany

Data and Applications

EAAC1 - SURFE2R 96SE

EAAC1 Surfe2r96SE

Icon 96SE SURFE2R 96SE data and applications:

EAAC1 pharmacology on the SURFE2R 96SE

Organic Cation Transporter 2 (OCT2) - SURFE2R 96SE

OCT2 SURFE2R 96SE

Icon 96SESURFE2R 96SE data and applications:

OCT2 transient currents recorded on the SURFE2R 96SE

 

PepT1 - Evoked signal via Glycylglycin

Surfer N96 PepT1 full screenIcon 96SE   SURFE2R N96 (a predecessor model of SURFE²R 96SE) data and applications:

Graphical user interface of screening software used on the SURFE2R N96. Traces of PepT1 recorded from membrane preparations of PepT1 overexpressing HEK293 cells are presented. The PepT1 signal is evoked by 20mM glycylglycin.

 

PepT1 - Pharmacology

Surfer N96 PepT1 Losarthan IC50 datacontrolIcon 96SE   SURFE2R N96 (a predecessor model of SURFE²R S96) data and applications:

Transport by PepT1 is inhibited by the kompetitive antagonist Losartan. When activated with 20 mM Gly-Gly, the IC50 of Losartan was 80.4 µM. Data were analysed with DataControl - the Analysis software tool for recordings on the SURFE2R N96 and SyncroPatch 96/ 384PE.

Downloads:

Product Sheets

SURFE2R 96SE - Product Sheet

Icon 96SE   SURFE²R 96SE product sheet   logo pdf   (1.7 MB)

Publications

2017 - SSM-Based Electrophysiology for Transporter Research

Icon N1   SURFE²R N1 and   Icon 96SE   SURFE²R 96SE book chapter in Methody in Enzymology

2017 - In vitro pharmacological characterization of the bispyridinium non-oxime compound MB327 and its 2- and 3-regioisomers

Icon 96SE  SURFE²R 96SE publication in Toxicology Letters (2017)

2016 - Functional analysis of Torpedo californica nicotinic acetylcholine receptors in multiple activation states by SSM-based electrophysiology

Icon 96SE   SURFE²R N96 (predesessor model of SURFE²R 96SE) publication in Toxicological Letters (2016)

2013 - Measuring Interference of Drug-Like Molecules with the Respiratory Chain: Toward the Early Identification of Mitochondrial Uncouplers in Lead Finding

Icon 96SE   SURFE²R N96 (predesessor model of SURFE²R 96SE) publication in Assay and Drug Development Technologies (2013)

Posters

2015 - "Studying Electrogenic Transporters of the SLC Family in a Parallel Way Utilizing Solid Supported Membrane Technology"

Icon 96SE   SURFE²R N96 poster (a predecessor model of the SURFE²R 96SE) Biophysics 2015   logo pdf   (0.8 MB)

2017 - An emerging technique for the characterization of transport proteins: SSM-based electrophysiology

Icon N1   SURFE²R N1 and    Icon 96SE   SURFE²R SE96 poster, 19th IUPAB / 11th EBSA congress 2017  logo pdf   (3.3 MB)

2017 - High throughput real-time measurement of electrogenic membrane transport driven by the SLC transporters PepT1 and OcT2

Icon 96SE   SURFE²R SE96 poster, Biophysics congress 2017  logo pdf   (1.8 MB)

 

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Nanion Technologies GmbH

Ganghoferstr. 70A
D-80339 Munich - Germany
info@nanion.de