Port-a-Patch publication in Biophysical Journal (2002)
Sigworth F.J., Klemic K.G.
Biophys. J. (2002) 82(6):2831-2832
To use one of the new benchtop patch-clamp systems, you first harvest the cells from a tissue-culture dish and wash by pelleting them in a microcentrifuge. Then you open the lid of the patch clamp box and pipette a few microliters of the cell suspension into the recording well. After a few minutes the cells settle onto the electrode array at the bottom of the well, the computer selects an electrode that has made a good seal, and the recording starts.