CaV2.2 | voltage-dependent, N type, alpha 1B subunit calcium channel

Family:
Calcium channels

Subgroups:
L-Type (CaV1.1–CaV1.4), P/Q-Type (CaV2.1), N-Type (CaV2.2), R-Type (CaV2.3), T-Type (CaV3.1–CaV3.3)

Topology:
Six transmembrane alpha helices (S1–S6), total of four homologous domains make up the tetrameric alpha subunit structure

Assembling:
One large alpha subunit forms a functional channel, accessory subunits ( α1, α2δ, β1-4, and γ) are crucial for robust expression, they functionally modulate the alpha subunit

CaV2.2: Background Information

CaV2.2 give rise to the N-type calcium currents and is distributed thourghout the entire body. These channels are known for their importance in the nervous system. They play a small role in the migration of immature neurons before the establishment of their mature synapses, and they are critically involved in the release of neurotransmitters, which is also similar to another type of calcium channels, known as P-type calcium channels. N-type calcium channels are targets for the development of drugs to relieve chronic and neuropathic pain. They are also used for the treatment of hypertension, Autism Spectrum Disorder, Osteoarthritis, and other medical diagnoses. Additionally, N-type calcium channels have known functions in the kidney, and heart.

Genes:
CACNA1B

Human Protein:
UniProt Q00975

Tissue:
brain, heart, lung, kidney

Function/ Application:
Neurotransmitter release in neurons, neurite outgrowth

Pathology:
Chronic pain, osteoarthritis, congenital generalized lipodystrophy, mental retardation, hypertension, diabetic neuropathy, Myoclonus-Dystonia syndrome, cancer, autism spectrum disorder

Interaction:
β1, β3, β4, α2δ, and possibly γ, laminin, ATP, RIMS1, FMR1, Amyloid Beta Precursor Protein Binding Family A Member 1, CaV2.1, CaV1.3, CaV3.1, CaV2.3, calmodulin, Guanine nucleotide binding protein gamma 4 and gamma 12

Modulator:
ω-Conotoxins, cadmium, gabapentin, nicardipine, TROX-1, ziconitide, (+-) Bay K 8644, cadmium

Assays:
Patch Clamp: whole cell, room temperature

Particularities:
CaV channels often show a rundown phenomenon. Adequate intra- and extrcellular solutions are essential for a good data quality.

Recommended Reviews:
Catterall WA, Perez-Reyes E, Snutch TP, Striessnig J (2005). "International Union of Pharmacology. XLVIII. Nomenclature and structure-function relationships of voltage-gated calcium channels". Pharmacol Rev. 57 (4): 411–25.

Data and Applications

Application Notes

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