Nanion Technologies

Orbit 16

Instant bilayers - just add protein.

Bilayer recording typically means tedious waiting for bilayer formation, protein introduction and eventually ion channel activity. The Orbit platforms take the pain out of bilayer recordings and enables fast and successful data generation thanks to the rapid formation of multiple bilayers and parallel recordings.

The Orbit 16 supports:

- Automated formation of 16 bilayers at the push of a button

- Low noise, high bandwidth recordings

- Various chip types for maximum experimental flexibility

- Substantial data generation through 16 simultaneous recordings

Orbit 16Most commercially available amplifiers are compatible with the Orbit 16, including the 16-channel Triton amplifier from Tecella. For single channel amplifiers like the EPC-10 or Axopatch, the Orbit has a built-in multiplexer switchboard, allowing for recordings from the individual bilayers by automatically switching through the recording sites.

Modular recording units

A complete Orbit 16 platform consists of the main recording station, an amplifier plus switchboard unit and two different recording modules supporting either Ionera's1 MECA-chip or Nanion's GUV-recording chips.

MECA-chipMECA – Micro Electrode Cavity Array (Ionera1)
The MECA recording substrate contains a 4 x 4 array of circular microcavities in a highly inert polymer. Each cavity contains an individual integrated Ag/AgCl-microelectrode. The bilayer is automatically formed by remotely actuated painting (Ionera1-SPREAD), thus roofing the liquid-filled cavity.

[1] Ionera (www.ionera.de), a spin-off from the University of Freiburg, Germany, will be the OEM for the MECA (microelectrode cavity array) chip.

GUV-chipGUV

As known from the Port-a-Patch, perforated borosilicate glass chips can successfully be used for low noise recordings from ion channels reconstituted in solvent-free bilayers. Here, bilayers are formed from giant unilamellar vesicles (GUVs) bursting over the 1 µm aperture contained in the borosilicate glass chip. The GUVs are captured by mild suction, and 16 (4 x 4 array) solvent-free bilayers are formed in parallel. Validation work is in progress.

 

Downloads:

Orbit 16 Product Sheet