• Orbit mini

    Simultaneous recording from four lipid bilayers

Orbit mini - Plug and paint

The Orbit mini is a minitiarized bilayer workstation enabling the recording of four artificial lipid bilayers simultaneously. The benefits the Orbit mini setup offers include:

  • Standalone turn-key system with extremely small footprint
  • Integrated four channel low-noise amplifier
  • Optional temperature control - automated active cooling and heating
  • Low noise recordings at highest bandwidths
  • Increased throughput due to four simultaneous recordings
  • Dedicated comprehensive recording software included
  • Target molecules can be introduced directly or by fusion of (proteo-)liposomes
  • Ion channels: voltage and ligand gated, temperature sensitive
  • Nanopores, antimicrobial peptides, toxins and many more...
  • Cost-efficient experiments with disposable MECA chips

The Orbit mini contains a built-in, miniaturized four-channel amplifier, allowing low noise recordings at high bandwidth without the need for any additional equipment – you could virtually conduct experiments while travelling by train! Due to the unique design of the Orbit mini, the measurement chamber temperature can be actively controlled without additional noise generation.

The complete Orbit mini platform consists of the main recording unit with a built-in four channel amplifier (Elements s.r.l.), and an optional computer-controlled environmental control unit for active cooling and heating of the recording chamber. Ionera's MECA 4 disposable recording chips are used for manual painting and recording of four artificial lipid bilayers in parallel.  

For detailed information:

Add-Ons and Features

Temperature Control Unit

With the temperature control unit, temperatures on the chip can be varied in between 0°C to 50°C, as it contains an active cooling and active heating function.

temperature control Orbit

Temperature control of the Orbit mini: PEG induced current blockages of a alpha-hemolysin pore

(A) Current traces recorded at 10°C and 40°C illustrating a strong increase of the open pore current as well as the blockage frequency at elevated temperature.
(B) Event averaged histograms of the residual current during blockages. The open pore current scales with the temperature as well as the dwell time of the blockages.
(C) Dependence of the open pore current on the temperature.
(D) Dependence of the frequency of blockages on the temperature.

The image war kindly provided by Ionera Technologies GmbH


Orbit mini Fluorescence Microscopy Kit

For fluorescence applications, the Orbit mini can be equipped with the "Fluorescence Microscopy Kit" which includes an adapted faraday shielding as well as "Meca 4 Recording Chips Fluo", four-well recording chips for fluorescence applications with 150 µm cavity size (Order # 132004).

Software

Orbit mini EDR Software

eFOUR Software

The software for the Orbit mini was developed by our partner Elements SRL (Italy). It is intuitive and easy to learn. Please watch this video on the Orbit mini to learn about the software:

16.07.2015 | Tutorial on Orbit mini

Icon Orbit Mini   Orbit mini

Short introduction on the Orbit mini (tutorial video, 7 minutes)


Software Updates:

Please download EDR Software Updates from the Elements s.r.l homepage, out partner for Orbit mini software tools. Click here for Software downloads.

Consumables

MECA 4 Recording Chips

The MECA recording substrate contains a 2 x 2 array of circular microcavities in a highly inert polymer. Each cavity contains an individual integrated Ag/AgCl-microelectrode. The bilayer is formed by painting, with high success rates for functional bilayers. The MECA chip has been validated with a number of different ion channels including KcsA, gramicidin, α-hemolysin, KV1.3, NaV and many more.

The MECA recording chips are produced and quality assured by our partner Ionera Technologies GmbH in Freiburg Germany and shipped from Munich to our international customers. Different types of MECA recording chips are available which should be chosen depending on experiment.

MECA 4 1


Available chip types
  • "Meca 4 Recording Chips 50 µm": Four-well recording chip with 50 µm cavity size (Order # 132001)
  • "Meca 4 Recording Chips 100 µm": Four-well recording chip with 100 µm cavity size (Order # 132002)
  • "Meca 4 Recording Chips 150 µm": Four-well recording chip with 150 µm cavity size (Order # 132003)
  • "Meca 4 Recording Chips Fluo": Four-well recording chip for fluorescence applications with 150 µm cavity size (Order # 132004)

Testimonials & Case Studies

Prof. Dr. Chris Miller - Case Study about the Orbit mini

Narrative Chris MillerIcon Orbit Mini   "Contrary to my initial expectations, the Orbit mini (red arrow) has performed exceptionally well for
our application, with vastly improved signal-tonoise characteristics over our best recordings on the home-built system."

“The compactness of the instrument makes for flexible and convenient placement in the lab. With a tiny footprint, it sits on a table or desk accompanied by only a laptop. In addition, bilayers are painted 'blind' with a teflon wand supplied by Nanion without observing the partition through a microscope. Thus, the
entire support-package of microscope, illuminator, Faraday cage, vibration-table, and rig-rack is eliminated. The acquisition software is intuitive and robust, and the data can be stored in formats readable by the Axon analysis programs that we routinely use. Moreover, the four electrically independent holes in the chip-partition means that four bilayers can be monitored simultaneously, thus quadrupling the probability of catching a clean single channel to record. This represents a significant, practical boost in experimental efficiency.”


Source: "Getting going with the Orbit mini planar bilayer system: A narrative"

From:
Prof. Dr. Chris Miller, Professor of Biochemistry, Investigator, Howard Hughes Medical Institute Member, US National Academy of Sciences, Brandeis University, Waltham, Massachusetts, USA

Read the full customer case study here

Data and Applications

Alpha-Hemolysin - Temperature Control

Icon Orbit Mini   temperature control OrbitOrbit mini and applications:
Data were kindly provided by Ionera.

Temperature control of the Orbit mini: PEG induced current blockages of a alpha-hemolysin pore

(A) Current traces recorded at 10°C and 40°C illustrating a strong increase of the open pore current as well as the blockage frequency at elevated temperature.
(B) Event averaged histograms of the residual current during blockages. The open pore current scales with the temperature as well as the dwell time of the blockages.
(C) Dependence of the open pore current on the temperature.
(D) Dependence of the frequency of blockages on the temperature.

OccK1 - Outer Membrane Protein of the Pathogenic Bacterium Pseudomonas Aeruginosa

Icon Orbit Mini   Orbit Mini OccK1 1Orbit mini and applications:
Data were kindly provided by Ionera.

Representative recording from a single OmpF trimer showing current blocks due to the translocation of antibiotic Enrofloxacin, event averaged histogram.
Conditions: 150 mM KCl, 5 mM MES, pH 6, 10 mM enrofloxacin, + 60 mV

OmpF - Current block

Icon Orbit Mini   Orbit Mini OmpF 1Orbit mini and applications:
Data were kindly provided by Ionera.

Representative recording from a single OmpF trimer showing current blocks due to the translocation of antibiotic Enrofloxacin, event averaged histogram.
Conditions: 150 mM KCl, 5 mM HEPES, 5 mM MES, pH 6, 10 mM enrofloxacin, +60 mV

TRPA1 - Temperature Dependency

Icon Orbit Mini   Orbit Mini TRPA1Orbit mini and applications:

(A) Effect of temperature on TRPA1 activity
(B) The open probability (Po) versus the temperature and fitted with Boltzmann equation (EC50 was found at 14˚C).

The Arrhenius plot of the same data resulted in a Q10 of 46 (Literature: Q10 ~ 40).

VDAC - Application of Fluoxetine

Icon Orbit Mini   Orbit Mini vdac 1Orbit mini and applications:
Data were kindly provided by Ionera.

Current recordings of single VDA channels from selected bilayers in parallel.
VDAC was reconstituted in DPhPC membranes and partially blocked upon the addition of Fluoxetine (Prozac).

(A) Representative parallel recording from 3 VDAC channels in DPhPC membranes. Conditions: 1 M KCl, 25 mM Tris, pH 7,5, voltage change protocol is given below.
(B) Current trace illustration partial blocade of VDAC by Fluoxetine (Prozac).
(C) Current-voltage trace of a single VADC channel under a ramping voltage of -70/+70 mV before (green) and after (blue) addition of 50 µm Fluoxetine

Purified VDAC sample is a generous gift from Prof. Stephan Nussberger to Ionera, Department Biophysics, University of Stuttgart, Germany

Webinars and Movies

Webinars

27.01.2016 | Webinar: Instant bilayers - just add protein.

Icon Orbit   Orbit 16 and   Icon Orbit Mini   Orbit Mini

Orbits V1 flat 250pxThis webinar covers the use of the lipid bilayer platforms from Nanion: the Orbit16 and the Orbit mini for characterization of membrane proteins like ion channels, bacterial porins and biological nanopores. Both bilayer systems support high quality low noise recordings, but differ in throughput capabilities and experimental features. The Orbit16, introduced in 2012 is a device for efficient formation of 16 lipid bilayers simultaneously, allowing for parallel bilayer-reconstitution of ion channels and nanopores.

 

Movies: Tutorials and Oral Presentations

16.07.2015 | How to prepare membranes on the Orbit mini

Icon Orbit Mini   Orbit Mini

Short introduction how to prepare membranes on the Orbit mini (tutorial video, 16 seconds)

29.07.2016 | Oral Presentation (Orbit mini, Prof. Dr. Chris Miller): A New Physiology to handle an ancient Challenge: Fluoride Resistance in Microorganisms

Icon Orbit Mini   Orbit mini

Chris Miller's talk presenting exciting data using the Orbit technology

16.07.2015 | Tutorial on Orbit mini

Icon Orbit Mini   Orbit mini

Short introduction on the Orbit mini (tutorial video, 7 minutes)

Downloads:

Application Notes

Product Sheets

Orbit mini - Product Sheet

Icon Orbit Mini   Orbit mini product sheet   logo pdf   (0.9 MB)

Quickstart Guides

Orbit mini - Quickstart Guide "Getting Started"

Icon Orbit Mini   Orbit mini Quickstart Guide "Getting Started"

Publications

2017 - Molecular determinants of permeation in a fluoride-specific ion channel

icon vpp   Orbit mini in eLife (2017)

2017 - Engineering a pH responsive pore forming protein

Icon Orbit Mini  Orbit mini and   icon vpp   Vesicle Prep Pro publication in Scientific Reports (2017)

2016 - Mechanistic signs of double-barreled structure in a fluoride ion channel

Icon Orbit Mini  Orbit mini publication in eLIFE (2016)

Posters

2016 - Next level toxicity screening: From single channel to overall cell behavior

Icon Orbit Mini   Orbit mini,   Icon CE   CardioExcyte 96 and   icon sp96   SyncroPatch 384PE poster, Meeting of the French Society of Toxinology (SFET) 2015  logo pdf   (0.9 MB)

 

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Nanion Technologies GmbH

Ganghoferstr. 70A
D-80339 Munich - Germany
info@nanion.de