• Port-a-Patch

    Smallest patch clamp setup in the world
  • Port-a-Patch

    Easy to learn - ideal for teaching
  • Port-a-Patch

    Records from cells, organelles and bilayers
  • Port-a-Patch

    First planar patch clamp device on the market
  • Port-a-Patch

    Ideal for internal solution exchange applications

Port-a-Patch: World's smallest patch clamp rig

The Port-a-Patch is a miniaturized patch clamp system supporting giga-seal recordings from one cell at a time. It offers fast and easy access to high quality patch clamp data with only minimal training. Not only a powerful research tool but also ideal for educational purposes and quick tests of cells and ion channels. The benefits the Port-a-Patch offers include:

  • External & internal perfusion
  • Temperature control
  • Voltage & current clamp
  • Whole-cell & perforated patch clamp
  • Single- & multihole chips, produced in-house
  • Rseries compensation
  • Unlimited compound applications
  • Voltage and ligand-gated ion channels
  • Cell lines, primary cells and stem cells
  • Easy to use, minimal training required
  • Adjustment of parameters during experiments for fast and efficient assay development

Using the Port-a-Patch is straightforward and easy – the user simply adds solutions and cells onto the disposable recording chip, where a cell is automatically captured and sealed by suction using a computer controlled pump. A microscope or micro-manipulator are not required.

Add-Ons such as the Internal and External Perfusion and Temperature Control, as well as the ability to conduct current clamp and bilayer experiments, broaden the range of possible experiments for scientific research immensely.

The Port-a-Patch system is comprised of the Port-a-Patch recording unit, a Suction Control unit, a HEKA amplifier and a computer. If an amplifier is already available, this can be integrated with the Port-a-Patch system in most cases. Further add-ons are available for specific assays as e.g. temperature control, microscope slides or automated perfusion systems.

For detailed information:

Add-Ons and Features

External Perfusion System

Products Pap external perfusion 330The External Perfusion System for the Port-a-Patch is comprised of a computer controlled magnetic pinch valve panel and a dedicated flow chamber. Up to eight different solutions can be handled by the perfusion panel. Solutions can either be switched manually or via the software. The latter allows synchronization of data acquisition with solution exchange, as well as tagging of the collected traces with relevant information such as compound and concentration applied. Temperature Control is also available in combination with the External Perfusion System enabling a stable maintenance of elevated temperatures.

The Laminar Flow Chamber which is provided with the External Perfusion System has a solution exchange time of <50 ms making it ideal for studies of both ligand and voltage gated channels.


Internal Perfusion System

products pap internal perfusion 330Nanion’s planar patch clamp chips offer the unique possibility to perfuse the intracellular side of the membrane. This aspect is exploited with the Internal Perfusion System for the Port-a-Patch. Up to eight different solutions can be perfused inside the cell, allowing dose response analysis of compounds acting on the cytosolic portion of the ion channel. Second messengers and metabolites can also be added internally to investigate how ion channel function is affected. Solutions are exchanged within seconds, and the ion channel modulation can be continuously monitored during the solution exchange.


products pap internal perfusion2 330The Internal Perfusion System is an Add-On for the Port- a-Patch. Combined with Nanion’s Perfusion System it can be operated either manually, or fully computer controlled. In this way, data acquisition can be synchronized with solution exchange and traces automatically tagged with the compound information.







Temperature Control

Products PaP Temp control 533

The Temperature Control for the Port-a-Patch allows experiments at elevated temperatures or application of short temperature pulses. Two components are used to heat the solutions: a canulla for heating the solution during continuous perfusion, and a resistive heating element integrated in the Faraday cage. This results in stable temperature maintenance whether or not the cell is perfused. A computer interface is used to monitor and control the temperature, allowing temperature changes with the click of a button.

Temperatures can be set up to 60 °C (± 0.5 °C) and are typically reached within two minutes. Cooling is passive. The Temperature Control is an Add-On for the Port-a-Patch and is a useful tool for studies of ion channels at physiological temperature and temperature gated channels, such as TRP channels.


Port-a-Patch Microscope Slide

microscope slide 2microsope slide 3Nanion offers the Port-a-Patch Microscope Slide developed for the simultaneous visualization and patch clamp recording of the cell. Fluorescence measurements using an upright microscope can therefore be combined with current recordings.


Suction Control Pro

Products Pap SuctionControl 330The Suction Control Pro contains an interface for communication with the analog in- and outputs of the patch clamp amplifier. Via this communication route, pressure application can be synchronized with voltage protocols and they can be simultaneously recorded.
The Suction Control Pro is ideally suited for studies of mechanosensitive ion channels and for studying the effect of pressure on artificial and cellular membranes. The add-on is available in conjunction with the Port-a-Patch. It is compatible with all existing Port-a-Patch systems.


Low Capacitance Holder

The Low Capacitance Holder for the Port-a-Patch has been developed to reduce the capacitance of the recording system, thereby increasing the temporal resolution and the signal-to-noise ratio. This reduction in capacitance is critical for experiments requiring increased resolution, e.g. recordings of small conductance ion channels with fast gating properties in a planar lipid bilayer or in the cell attached mode. The capacitance of the whole system including a bilayer on the chip is approximatey 2.5 pF, compared to 6.5 pF for the standard Port-a-Patch. This corresponds to Irms values of 400 fA compared to 1.1 pA (gain: 1000 mV/pA, bandwidth: 3 kHz) for the Low Capacitance Holder and the standard Port-a-Patch.

PatchControl Software

PatchContol: the intuitive Software of the Port-a-Patch


PatchControlPatchControl is a software programme written with the user in mind. Pre-written protocols can be loaded and altered, allowing the entire procedure for cell capture, sealing and obtaining the whole cell configuration to be completely automated. However, because we know that sometimes you just want to control things yourself, the protocols can be interrupted at any time and the suction controlled manually.

Through innovative communication with electrophysiology software, PatchControl can read important parameters such as membrane resistance, whole cell capacitance and series resistance and moves through the pre-programmed steps accordingly. Parameters such as suction strength and duration, voltage and membrane criteria can be set and altered to obtain the optimum parameters for cells routinely used in your lab. Not only that, complex macros can be easily implemented, allowing everything from simple changes in gain to complex perfusion routines to be programmed and automatically performed.

The PatchControl software is there to make your patch clamp life easier, from automated cell capture to automated pressure control, the PatchControl software can do it all!

Consumables

NPC-1

Products NPC1 330

The NPC-1 chip is a proprietary innovative product by Nanion Technologies, developed for the Port-a-Patch. It is produced and quality-assured in-house at Nanion headquarters and shipped from Munich to our international customers. Different types of NPC-1 chips are available which should be chosen depending on cell size and application.


Material
The core structure of the NPC-1 chip is a planar borosilicate glass slide with its micron-sized patch aperture. This structure is incorporated into a screw cap for easy handling and minimal cycle times between experiments. Additionally, the unique design of the NPC-1 chip gives similar access to the external and internal solution. Continuous perfusion of the internal solution has finally become possible.
Available chip types
  • "NPC-1, 1-2 MOhm": (Order # 061101)
  • "NPC-1, 2-3.5 MOhm": (Order # 061102)
  • "NPC-1, 3-5 MOhm": (Order # 061103)
  • "NPC-1, 5-10 MOhm": (Order # 061104)
  • "NPC-1, 8-12 MOhm": (Order # 061105)
  • "NPC-1, 10-15 MOhm": (Order # 061106)

Other sizes are available upon request!

Buffers and Solutions

Buffers and Solutions for the Port-a-Patch

The buffers and solutions for the Port-a-Patch are produced by Nanion and an external partner, quality-assured in-house at Nanion headquarters and shipped from Munich to our international customers.


Available buffers and solutions
  • "Reagent Kit Perfusion System standard; external": 12x 50 mL external standard (Order # 081002)
  • "Reagent Kit Perfusion System standard; internal": 10x 50 mL internal (Cs), 10x 50 mL internal (K) (Order # 081019)
  • "Reagent Kit Perfusion System to be specified by the customer": 1x rack (20x 50 mL) (Order # 081015)
  • "Reagent Kit Port-a-Patch standard": 27x 1 mL external standard, 18x 1 mL internal standard (Cs), 18x 1 mL internal standard (K) (Order # 081001)
  • "Reagent Kit Port-a-Patch to be specified by the customer": rack with 81 x 1 mL vials (K) (Order # 081016)
  • "Reagent Kit Vesicles for Port-a-Patch": 18x 200 mM KCl (pH 7.0), 18x 200 mM KCl (pH 4.0), 18x 200 mM NaCl (pH 7.0), 18x 200 mM NaCl (pH 4.0), 7x 1M Sorbitol, 1 mM EGTA (pH 7.0), 1x GUVs, 1x 100 mM HCl (Order # 081006)

Data and Applications

Alamethicin - Bilayer Recordings

application alameticin 2icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:
Data were kindly provided by M. Sondermann/Prof. Behrends, Univ. Freiburg.

Alamethicin is a channel forming peptide and, when patch clamped, reveals multiple non-equidistant conductance levels due to formation of alamethicin oligomers in the bilayer. Alamethicin single channel conductances. Recordings from a GUV prepared bilayer in 85 mM KCl at -140 mV.

Alpha-Hemolysin - DNA Translocation

p45 4 AlphaHemolysinNeu icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:

Data were kindly supplied by Prof. Fritz Simmel, Technical University of Munich, Munich, Germany.

Reconstituted Alpha hemolysin channels are constantly open at positive and negative membrane potentials. Gating is observed as a result of the passage of a single stranded DNA molecule through the pore. Recordings were performed on the Port-a-Patch.

Bilayer Recordings - Low Noise

application Low noise holdericon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:

Typical recording of the Irms noise level of a bilayer (DPhPC) on the Low Capacitance Holder. Typical values are 220 fA

(10 kHz filter frequency, 50 kHz sampling rate. Cf. 2.3 pA RMS on the standard Port-a-Patch).

 

BY2 Protoplasts - Current Traces

by2cells 2icon pap   Port-a-Patch data and applications:

Tobacco (Nicotiana tabacum L. cv Bright Yellow 2 [BY2]) cells are the most widely used plant cell culture. Depolarisation-activated outward currents in BY2 cell protoplasts. Shown are whole cell current in response to voltage steps (left) and the corresponding IV curve (right). From a holding potential of −40 mV, 500 ms voltage steps were applied from −60 mV to 60 mV in 20 mV increments, with 3 s intervals.

Cardiac Action Potentials - Cor.At® Cells

p14 2 CorAT

icon pap   Port-a-Patch data and applications:
Cells were kindly provided by Axiogenesis

Cor.At® cardiomyocytes are derived from mouse embryonic stem (ES) cells. Whole cell currents recorded in voltage clamp mode reveal cardiomyocyte-typical ion channels (K+, Ca2+ and Na+). Traces on the lower left show prolongation of the action potential upon application of Dofetilide (1 μM).

Download: Application Note

Cardiac Action Potentials - hESC-derived Cells

p14 3 hESC

icon pap   Port-a-Patch data and applications:
Cells were kindly provided by Geron/GE.

The left picture shows a typical action potential in hESC-derived cardiomyocytes (human embryonic stem cell-derived cardiomyocytes). The traces represent K+- (top left), Ca2+- (bottom left) and Na+-currents (top right). The lower right panel shows the corresponding I/V-relationships.

Cardiomyocytes (Ventricular Myocytes) - Recordings

VentricularMyocyte1

icon pap   Port-a-Patch data and applicatons:
Thanks to S. Rakovic and D. Terrar from University of Oxford, for preparing the cardiomyocytes used in these experiments.

Ca2+ current recorded from a ventricular myocyte. Raw current voltage relationship and peak current data are plotted (top). From the IV-plot the half-activating voltage was determined as -16 mV. The current was later blocked by the L-type Ca2+ channel blocker, nifedepine (10 µM). Currents were elicited by stepping to +10 mV for 200 ms from a holding potential of -40 mV every 5 seconds.

CaV2.2 - Current-to-Voltage Relationship

Cav22IV PaP

icon pap   Port-a-Patch data and applications:
Cells were kindly provided by Millipore.

Representative current responses of an individual HEK-293 cell expressing CaV2.2 to a standard voltage protocol. Average current-voltage relationship (n = 10). The error bars reflect the standard error of the mean (S.E.M.).

CaV3.2 - Channel Blockers

Cav32 Block

icon pap   Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx Millipore.

Shown are raw current traces (top) and average dose response curves (bottom) of CaV3.2 (T-type Ca2+- Channel) block by compounds as indicated. CaV3.2 is stably expressed in HEK293 cells. IC50s were 863 nM (Mibefradil), 27 μM (Nifedipine) and 52 μM Amiloride.

 

 

 

 

CaV3.2 - T-Type Calcium Channels

Cav32 IV

icon pap   Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx Millipore.

Shown are raw current traces (top left) and average peak current data (top right) of the current voltage relationship of CaV3.2 (T-type Ca2+- Channel) stably expressed in HEK293 cells. Activation and Inactivation plots were constructed (bottom). Half-activation and half-inactivating potentials were determined as -32 mV and -65 mV, respectively.

 

 

Chloride Channels - Lysosomes Recordings

Lysosomes bothicon pap   Port-a-Patch data and applications:
Data are taken from Schieder M. et al., The Journal of Biological Chemistry, 2010, 285(28), 21219-21222.

Typical chloride currents in four isolated lysosomes.Chloride currents were determined as difference currents between IV curves recorded in the absence and presence of extralysosomal high (64 mM) Cl-. On the left a sketch is illustrating the methodology.

CNG - cAMP-Regulation of a Plant Ion Channel

p15 4 cAMP

icon pap   Port-a-Patch data and applicatons:
Data were kindly provided by A. Kugler & P. Dietrich, Univ. Erlangen, Germany.

The image shows internal cAMP-activation of a transiently expressed plant CNG channel and subsequent external block by lanthanum. Activators and blockers were manually added to the intracellular and extracellular sides of the membrane.

Cytolysin (Bacterial) - Bilayer Recordings

application bacterial 3icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:
Recordings were kindly supplied by ITC & CNR-Istituto di Biofisica, Trento, Italy.

Traces were recorded in 100 mM KCl, 10 mM Hepes, 0.1 mM EDTA, pH 7 at -40 mV. Lipid: Diphtanoyl-PC.

 

Endoplasmatic Reticulum Membrane Protein Translocator (Sec61) - Single Channel recordings

Sec61 Rampicon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:
Data were kindly provided by A. Cavalié, M. Jung, R. Zimmermann, Universität des Saarlandes, Homburg, Germany.

Microsomes were fused with GUVs within the VesiclePrepPro. The resulted Proteoliposomes formed a bilayer onto the NPC-1 Chip of the Port-a-Patch. Nice single channel openings could be recorded in symmetrical KCl solution applying a ramp from -110 mV to +100 mV for 400 ms.

Erythrocytes - Single Channel Analysis

application erythro 1

icon pap   Port-a-Patch data and applications:

Erythrocytes lack mitochondria and nuclei and consist mainly of hemoglobin. The membrane contains different ion channels, for example, a Ca2+-activated K+ channel and a volume-sensitive Na+/K+ pump. Here, single channel activity recorded from an erythrocyte in the cell attached configuration is shown.

GABAA Receptor (a1b2g2) - Reliable Compound Application

p20 2 GABAicon pap   Port-a-Patch data and applications:

Ligand dependent activation of GABAA receptors can be recorded with the Laminar Flow Chamber with approx. 100 ms time constant for 10 μM GABA. Concentration dependent activation and desensitization of GABAA (α1β2γ2) receptors was obtained by applying 1, 3 and 10 μM GABA for 2.5 s at a time interval of 20 s.

Glycine Receptor (GlyRa1) - Pharmacology

p20 3 hGlyRalpha1icon pap   Port-a-Patch data and applications:
Cells were kindly provided by AstraZeneca, Sweden.

The Laminar Flow Chamber can also be used for studying glycine receptors. The pharmacology of the hGlyRα1, expressed in a mouse fibroblast cell line (L-tk), was investigated. The EC50 for glycine was determined as 89 ± 2.7 μM (n = 10) which is in accordance with the literature.

Gramicidin - Single Channel Analysis

application gramicidin 1icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:
Recordings were kindly supplied by Tohoku University, Tohoku, Japan.

Plotting the current amplitude vs. the voltage reveals conductances of 94.88 pS and 28.28 pS, which correspond to two different gramicidin derivates present in the bilayer. Traces were recorded in 100 mM HCl at the indicated potentials. Clearly two gramicidin derivates (94.88 pS and 28.28 pS) can be distinguished.

Gramicidin - Rapid Internal Solution Exchange

p24 4 InternExchicon pap    Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:

Switching of internal solutions during gramicidin recordings from a lipid bilayer was obtained within seconds. A lipid bilayer was formed using giant unilamellar vesicles. Currents were recorded at a holding potential of +150 mV. The internal solution was switched from HCl to KCl, resulting in lower channel conductance.

hEAG (hKv10.1) - Internal Application

application heag 1

icon pap   Port-a-Patch data and applications:
Data were kindly provided by Walter Stühmer, MPI for exp. Med., Göttingen.

Block of hEAG whole cell currents after the internal application of 200 µM TEA.

 

hERG - Good Results with "Sticky Compounds"

p14 1 hERG

icon pap   Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx/Millipore.

Sticky compounds, such as some hERG blockers, exhibit expected IC50 values with the Port-a-Patch. The concentrations of the half maximal block were: 1.27 nM (astemizole), 8.9 nM (cisapride), 163.7 nM (flunarizine) and 11.0 nM (terfenadine).

hERG - Pharmacological Experiments at 35 °C

p28 4 fullDoseRespicon pap   Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx/Millipore, UK.

A full dose response curve of quinidine acting on the hERG channel was obtained at physiological temperature (35 °C). The IC50 for quinidine at physiological temperature was 1.3 ± 0.2 μM (n = 5), similar to that obtained at room temperature (1.0 ± 0.03 μM, n = 3).

hERG - Temperature Control

DATA Temp Control small

icon pap   Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx/Millipore.

The image shows example traces for hERG mediated currents at 25 ± 2 °C (black) and 35 ± 2 °C (blue). The peak current amplitude was increased at 35 °C, and the rise time and decay time constants were faster at physiological temperature compared with those obtained at room temperature. 

IMR32 Differentiated Cells - Sodium and Potassium Currents

Cori IMR32 Seite 61 67

icon pap   Port-a-Patch data and applications:

Whole cell current responses of undifferentiated (left) and for 12 days differentiated (right) IMR32 cells to a voltage step protocol (holding −90 mV, stepping for 20 ms from −60 mV to +60 mV in 20 mV increments) are shown. The change in current shape indicating increased expression of KV and NaV are apparent.

KCa1.1 (BK) - Intracellular Second Messengers

icon pap   Port-a-Patch data and applications:p24 2 InternalApp

Currents mediated by BK channels expressed in CHO cells were studied using the Internal Perfusion System. Currents were elicited by a voltage step from -80 mV to +80 mV before and after adding an internal solution containing a higher concentration of free Ca2+.

KCa1.1 (BK) - Single Channel Recordings

application kca11 1

icon pap   Port-a-Patch data and applications:

Single channel recordings of BK channels as recorded from CHO cells in the cell attached mode at +60 mV (top), +40 mV (middle) and 0 mV (lowest trace).

 

Kir Channels - Response to Different External Potassium Concentrations

application kir2 1neuicon pap   Port-a-Patch data and applications:

Current response in RBL cells to a voltage ramp from –150 mV to +80 mV in the presence of a low and a high external K+ concentration (left). Voltage dependent block of the inward K+ current after the external additon of 50 µM Ba2+ (middle). Same block in response to a continuous voltage step to –150 mV (right) from 0 mV.  Low K+ (4.5 mM K+), high K+ (143 mM K+).

KV1 (Shaker-Relared Potassium Channels) - Block of Mutated Channel

application shaker 2icon pap   Port-a-Patch data and applications: 
Data were kindly provided by Dr. Kenton Swartz, NIH, Bethesda, USA.

Block of Shaker-IR by Agitoxin2. Shown are current recordings from HEK293 transiently expressing Shaker-IR. Currents were elicited by a 500 ms step to +50 mV from a holding potential of -80 mV. K+ gradients are the same as for the IV curve experiment shown above. 200 nM Agitoxin2 almost fully blocks the Shaker K+ current.

KV1 (Shaker-related Potassium Channels) - Mutation without Inactivation

application shaker 1icon pap   Port-a-Patch data and applications: 
Data were kindly provided by Dr. Kenton Swartz, NIH, Bethesda, USA.

Here we show recordings from HEK293 cells transiently expressing a Shaker K mutant that has its inactivation removed (Shaker-IR). The holding potential was -80 mV. The current-voltage relationship of the tail currents reveals a half-activating potential of -27 mV.

KV1.3 - Continuous Internal Perfusion

p24 1 internalPerf

icon pap   Port-a-Patch data and applications:

KV1.3 currents (blue), endogenously expressed in Jurkat cells, were rapidly blocked by internal perfusion of Cs+ (light blue), and fully recovered after washout with K+ (grey). Internal solution replacement was repeated 19 times and the recording was stable for over 35 minutes, as shown in the lower graph.

KV1.3 - Internal Application of TEA and Quinidine

icon pap   Port-a-Patch data and applications:p25 1 Kv1,3

KV1.3 current was blocked by the internal application of increasing concentrations of quinidine (left) or TEA+ (right). For quinidine the IC50 was determined as 15 μM (literature value external application 14 μM) and for TEA+ the IC50 was determined as 0.9 ± 0.3 mM (n = 3) (literature value internal application 0.6 mM).

KV1.3 - Perforated Patch

application hkv13 1application hkv13 2

icon pap   Port-a-Patch data and applications:

Perforated and conventional whole cell configuration derived hKV1.3-currents. Voltage-dependence was shifted significantly to more negative potentials in the whole cell configuration compared to the perforated whole cell configuration. Whole cell currents after a conventional membrane breakthrough (right) and perforated whole cell currents (left).

KV1.3 - Voltage Induced Membrane Movements Measured with Atomic Force Microscopy

icon pap   Port-a-Patch data and applications:Porti AFM JurkatWebData are taken from Pamir E., et al., Ultramicroscopy, 2008, 108, 552-557.

Voltage induced membrane movements of a Jurkat cell. (a) Deflection signal of the cantilever resting on the cell membrane at an indenting force of 1.0 nN. (b) Corresponding whole cell current: The characteristic response of the voltage gated potassium channel KV1.3 in Jurkat cells is observed. (c) Below: Applied pulse protocol.

Mitochondria - Cell Attached Recordings

application mitochondria 1icon pap   Port-a-Patch data and applications:

Mitochondria were isolated from rat cardiac tissue. The patch clamp measurements were performed in the cell attached configuration with an Axopatch 200B. Single channel currents were elicited by a voltage ramp from 0 mV to -80 mV. Due to the cell attached configuration inward currents are displayed as a positive current.

MscL - Pressure Clamp Experiments

p16 2 MscLicon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:

The Port-a-Patch and the Suction Control Pro was used to study the effect of pressure on functional MscL protein reconstituted in GUVs. Using symmetrical KCl solutions, MscL gating was observed at -30 mBar and -60 mBar (holding potential + 30 mV), shown as outward currents in the data traces.

NaV1.5 - Accurate Voltage Control

p14 4 Nav

icon pap   Port-a-Patch data and applications:
Cells stably transfected with human SCN5A were kindly provided by Millipore.

To perform recordings of fast events, such as the activation and inactivation of Na currents, it is essential to have accurate voltage control. The image shows currents and I/V characteristics of NaV1.5 expressed in HEK293 cells.

NBC1 (Electrogenic Na+/HCO3- Cotransporter) - Current responses

application kncb1 1icon pap   Port-a-Patch data and applications:
Data were kindly provided by Ira Kurtz, UCLA, USA.

Recordings from HEK 293 cells expressing the electrogenic Na+/bicarbonate cotransporter (NBC1). Current responses to 200 ms voltage ramps in high external Na+ initially after establishment of the whole cell configuration (control), after application of HCO3- (bicarbonate), and after wash-out (wash-out).

Neural Cells (Primary Mouse Stem Cells) - Current Traces

p15 2 Primary Stem 2icon pap   Port-a-Patch data and applications:
Data courtesy of Dr. Gavin Dawe, National Uni­versity of Singapore.

Patch clamp recordings from cultured mouse primary neuronal stem cells were made in the whole cell configuration (holding potential −60 mV). Currents were evoked by 500 ms depolarizing voltage pulses, showing outwardly rectifying K+- currents.

Neurons (Primary Hippocampal Granule Cell) - Current Traces

icon pap   Port-a-Patch data and applications:

Recordings from acutely isolated hippocampal granule cells show BK- and CaV currents. Whole cell currents were obtained by depolarizing voltage pulses from a holding potential of −80 mV.

P2X3 - Application of ATP

PortiP2X3icon pap   Port-a-Patch data and applications:
Cells were kindly supplied by Evotec AG, Hamburg, Germany

Shown are raw current traces from 1321 N1 cells expressing P2X3 receptors demonstrating the reproducability of the whole cell responses. Currents were activated by 100 ms application of 10 µM ATP. The waiting time between sweeps was 120 s (holding −60 mV).

Potassium Channels - Currents Measured in Differentiated IMR32 Cells

Cori IMR32 Seite 64 70icon pap   Port-a-Patch data and applications:

Whole cell current responses of undifferentiated (a) and for 12 days differentiated (b) IMR32 cells to a voltage step protocol (holding −90 mV, stepping for 500 ms from −60 mV to +60 mV in 10 mV increments) are shown. In (c) and (d) the corresponding current voltag relationships are shown (closed circles). Open circles are the correspondig current voltage relationships after the cells have been exposed to internal Cs+.

Protoplasts (Mesophyll Tabacco Leaves) - Hyperpolarization-Activated Inward Currents

mesophyll cells 2icon pap   Port-a-Patch data and applications:
Data were kindly provided by Berghöfer; Eing; Flickinger; Frey (Forschungszentrum Karlsruhe, Germany).

Mesophyll protoplasts are enzymatically isolated from tobacco leaves. Shown are whole cell recordings which demonstrate the hyperpolarization-activated inward currents in the protoplasts. A voltage ramp from −40 mV to −120 mV was applied.

Sonoporation Technology - Contrast Agent Combined with Ultrasound

application sonoporation 2icon pap   Port-a-Patch data and applications:
Data were kindly provided by Dr. Cheri Deng, University of Michigan, USA.

Current response of a non-transfected Chinese hamster ovary (CHO) cell to a train of ultrasound pulses (US) in the absence (A) and presence (B) of contrast agent. The holding potential was -80 mV. Ultrasound signals had a centre frequency of 5 MHz. Pulses of 50 cycles were repeated for 2 s every 10 ms.

TRPM7 - Current Traces

mTRPPM7InternalPerfPorty smlicon pap   Port-a-Patch data and applications:

Shown are mTRPM7 (HEK293) raw current responses to a voltage ramp voltage procotol  (−100 mV to 100 mV over 200 ms) recorded under control conditions and after subsequent internal perfusion with Mg2+ (left). Initally, currents showed some run-up before they became stable. The arrows in the timecourse on the right mark the timepoints for which the raw current traces are shown.

TRPM8 - Activated by Menthol

AN Port a Patch TRPV1M8 2icon pap   Port-a-Patch data and applications:

External application of 300 µM menthol activated the TRPM8 channels expressed in HEK293 cells.

 

 

 

TRPM8 - Bilayer Recording

TrpM8 420x420icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications: 
Data kindly provided by Dr. Zakharian and Dr. Rohac From UMDNJ New Jersey Medical School, Newark, NJ 07103, USA

Shown are single channel events of TRPM8 reconstituted in a planar lipid bilayer. The recordings were made with the with the Port-a-Patch. The channel was activated by PIP2 and methanol. Currents were recorded at 100 mV.

TRPM8 / TRPV1 - Pharmacology

icon pap   Port-a-Patch data and applications:p15 1 TRP 
TRPV1 data were kindly supplied by Dr. David Cohen, Oregon Health and Service University, Portland, OR, USA.

TRP channels recorded in HEK293 cells. Menthol (dark blue) activated the TRPM8 channels (shown on the left) and could be inhibited by genistein (blue). TRPV1 channels expressed in HEK293 cells (right) were stimulated by application of capsaicin.

TRPV1 - External Perfusion of Capsaicin

application trpv1 1icon pap   Port-a-Patch data and applications:
Data were kindly provided by David Cohen, Oregon Health & Science University, Portland, USA.

Whole cell current responses from HEK 293 cells transiently expressing TRPV1 to a ramp voltage protocol (‑100 mV to +100 mV). Capsaicin at a concentration of 2 μM reversibly activated the channel.

TRPV1 - Heat Activation

p28 2 heatActivChanicon pap   Port-a-Patch data and applications:

Raw traces of TRPV1 current responses to voltage ramps from –100 mV up to +100 mV. TRPV1 current at RT (28°C), during two stimulations by application of heated solution (34°C and 36°C), and after cooling down to RT (29°C). The picture on the right-hand side shows the current amplitudes as recorded at -100 mV and +100 mV plotted against time.

TRPV1 - Internal Application of CaM

icon pap   Port-a-Patch data and applications:p24 3 TRPV1

TRPV1 currents were elicited by the external application of 20 μM capsaicin. After capsaicin activation, the currents were partially blocked by the internal application of Ca2+-calmodulin (50 μM Ca2+ / 500 nM CaM). TRPV1 channels were expressed in CHO cells.

 

Testimonials & Case Studies

Dr. Chris Fanger - Statement about the Port-a-Patch

icon pap   “The Port-a-Patch is a state-of-the-art automated patch clamp  platform that allows trainees at Hydra without prior experience in  electrophysiology to quickly learn how to generate high quality patch clamp recordings. It substantially speeds up the teaching process as well as giving us an easy-to-use platform for efficient accurate screening of ion channel active compounds.”

Chris Fanger, Director of Lead Discovery
Hydra Biosciences, Boston, MA, USA

Dr. Marisa Rangel - Statement about the Vesicle Prep Pro and Port-a-Patch

icon vpp   icon pap   “Our planar lipid bilayers are obtained from GUVs prepared with the Nanion Vesicle Prep Pro setup. Together with the Port-a-Patch, Nanion´s state-of-the-art automated patch clamp platform, the efficiency of characterization of pore-forming peptides (and other ionophores from natural sources) in artificial bilayers has greatly increased. Both systems are straightforward and easy to use, allowing high quality, low noise, single channel recordings.”

Marisa Rangel, PhD, Researcher 
University of Sao Paulo, Butantan Institute, Sao Paulo, Brazil, WI, US

Prof. Dr. Armin Kargol - Statement about the Port-a-Patch

icon pap   “Nanion’s Port-a-Patch is an excellent instrument for research labs working with undergraduate students. Manual patch-clamping methods depend to a large extend on the skills of the operator. They require long training periods. For a typical undergraduate student it takes over a year to learn patch-clamping. Port-a-Patch reduces that time to only weeks and the most difficult part is to understand the software that controls the instrument. This allows undergraduate students to actively participate in real research projects.”

Prof. Dr. Armin Kargol, Professor of Physics, Rev. James C. Carter, S.J., Distinguished Professor in Experimental Physics, Department Chair
Loyola University, Louisiana. USA

Prof. Dr. Carlos Villalobos - Statement about the Port-a-Patch

“We have used Nanion´s Port-a-patch for recording endogenous Icrac, the small calcium-release activated currents that are becoming very important in cell fate and cancer hallmarks. In addition, measurements were carried out in primary cultures of human, coronary, vascular-smooth muscle cells, a very difficult cell type to patch, and several normal and tumoral, epithelial cells. In the beginning I was very skeptical, because the currents are extremely small. However, the results were excellent and clear cut. It is possible to investigate the pharmacology and molecular biology of these channels during the phenotypic switches related to transdifferentiation, aging or tumorogenesis”.

Prof. Carlos Villalobos
University of Valladolid and Spanish Research Council (CSIC), Spain

Prof. Dr. Colin Taylor - Statement about the Port-a-Patch and Vesicle Prep Pro

icon vpp   “We are using the Vesicle Prep Pro and Port-a-Patch in our laboratory to study recombinant channel proteins reconstituted in planar bilayers. The system is easy to use and it makes experiments very efficient. Besides the great equipment, Nanion, with their widespread expertise, is also providing excellent continuous support, helping with methodology optimization and problem solving.”

Colin Taylor, Professor of  Cellular Pharmacology, Department of Pharmacology
University of Cambridge, Cambridge, UK

Prof. Dr. Friedrich Simmel - Statement about the Port-a-Patch

icon pap   “The Port-a-Patch enables students and young researchers lacking formal training in electrophysiology to rapidly generate research grade data. ­Within ­the ­courses ­given ­at ­the ­Institute ­for Biomolecular ­Systems and ­Bioelectronics, ­the ­students­ gain ­hands-on­e xperience­ in ­basic membrane ­biophysics ­and ­electrophysiology,­ which­ is ­of ­great ­value for their education.”

Professor­ Dr. Friedrich Simmel
­Technical­ University ­of ­Munich, ­Munich, ­Germany

Webinars

07.10.2015 | Webinar: Patch Clamp Made Easy, Fast Track to Excellence

icon pap   Port-a-Patch

This webinar shows applications that go way beyond possibilities of conventional patch-clamping, where the Port-a-Patch facilitates completely novel scientific directions.

Downloads:

Application Notes

Bacterial Spheroplasts - "Preparation and recordings on the Port-a-Patch of native E. Coli spheroplasts"

icon pap   Port-a-Patch application note:   logo pdf   (0.5 MB) 

Cardiomyocytes - "Action Potentials in Mouse ES Cell-Derived Cor.At Cardiomyocytes on Nanion´s Port-a-Patch"

icon pap   Port-a-Patch application note:   logo pdf   (0.6 MB)
Cells were kindly provided by Axiogenesis.

CaV2.2 - "Characterization of CaV2.2 on Nanion's Port-a-Patch"

icon pap   Port-a-Patch application note:   logo pdf   (0.6 MB)
Cells were kindly provided by Millipore.  

CaV3.2 - "Characterization of CaV3.2 on Nanion's Port-a-Patch"

icon pap   Port-a-Patch application note:   logo pdf   (0.7 MB)
Cells were kindly provided by Millipore.  

hERG - "Characterization of hERG (CHO) on Nanion's Port-a-Patch "

icon pap   Port-a-Patch application note:   logo pdf   (0.6 MB)
Cells were kindly provided by Millipore.  

hERG - "Characterization of hERG (HEK293) on Nanion's Port-a-Patch"

icon pap   Port-a-Patch application note:   logo pdf   (0.7 MB)
Cells were kindly provided by Millipore.  

hERG - "Pharmacology of hERG recorded on Nanion´s Port-a-Patch"

icon pap   Port-a-Patch application note:   logo pdf   (0.4 MB)
Cells were kindly provided by Millipore.  

hERG - "Temperature controlled hERG recordings on the Port-a-Patch"

icon pap   Port-a-Patch application note:   logo pdf   (0.5 MB)
Cells were kindly provided by Millipore.  

KcsA - "Lipid bilayer recordings of KcsA channels reconstituted in proteoliposomes"

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro application note:  logo pdf   (0.2 MB)

KV1.3 -Port-a-Patch - "Automated internal perfusion of Jurkat cells expressing KV1.3 channels"

icon pap   Port-a-Patch application note:   logo pdf   (0.2 MB) 

Mitochondria - "Mitoplasts from HEK cells on Nanion‘s Port-a-Patch"

icon pap   Port-a-Patch application note   logo pdf   (0.5 MB)

Mitochondria - "Recordings from mitochondria and mitoplasts"

icon pap   Port-a-Patch application note   logo pdf   (0.4 MB)

MscL - "Lipid bilayer recordings of a mechanosensitive channel, MscL, using Nanion’s pressure clampchannel, MscL, using Nanion’s pressure clamp"

icon pap   Port-a-Patch and    icon vpp   Vesicle Prep Pro application note   logo pdf   (0.3 MB)

NaV1.7 - "Patch clamp recordings of hNaV1.7 on Nanion’s Port-a-Patch"

icon pap   Port-a-Patch application note   logo pdf   (0.7 MB)
Cells were kindly provided by Anaxon

OmpF - "Lipid Bilayer recordings of OmpF reconstituted in Proteoliposomes "

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro application note:   logo pdf   (1.4 MB) 

TRPA1 - "TRPA1 activation by allyl isothiocyanate recorded on the Port-a-Patch"

icon pap   Port-a-Patch application note:  logo pdf   (1.4 MB)

TRPM7 - "TRPM7 activation by internal sequestering of Mg2+ ions recorded on the Port-a-Patch"

icon pap   Port-a-Patch application note:  logo pdf   (0.8 MB)

TRPM8 / TRPV1 - "TRPV1 and TRPM8 recorded on Nanion's Port-a-Patch"

icon pap   Port-a-Patch application note:  logo pdf   (0.2 MB)

Product Sheets

Port-a-Patch - Product Sheet

icon pap   Port-a-Patch product sheet:   logo pdf   (0.6 MB)

Port-a-Patch Product Flyer - External Perfusion

icon pap   Port-a-Patch product flyer   logo pdf   (1 MB)

Port-a-Patch Product Flyer - Internal Perfusion

icon pap   Port-a-Patch product flyer   logo pdf   (0.8 MB)

Port-a-Patch Product Flyer - Suction Control Pro

icon pap   Port-a-Patch product flyer   logo pdf   (0.2 MB)

Port-a-Patch Product Flyer - Temperature Control

icon pap   Port-a-Patch product flyer   logo pdf   (0.8 MB)

Publications

2017 - Polydim-I antimicrobial activity against MDR bacteria and its model membrane interaction

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in PLoS ONE (2017)

2017 - Peptidomic analysis of the venom of the solitary bee Xylocopa appendiculata circumvolans

icon pap   Port-a-Patch publication in Journal of Venomous Animals and Toxins including Tropical Diseases (2017)

2017 - Mitochondria sustain store-operated currents in colon cancer cells but not in normal colonic cells: reversal by non-steroidal anti-inflammatory drugs

icon pap   Port-a-Patch publication in Oncotarget (2017)

2017 - Evaluation of the antimicrobial activity of the mastoparan Polybia-MPII isolated from venom of the social wasp Pseudopolybia vespiceps testacea (Vespidae, Hymenoptera)

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in International Journal of Antimicrobial Agents (2017)

2017 - Bilayer-Mediated Structural Transitions Control Mechanosensitivity of the TREK-2 K2P Channel

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Structure (2017)

2017 - Asymmetric mechanosensitivity in a eukaryotic ion channel

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in PNAS (2017)

2016 - Voltage Dependence of Conformational Dynamics and Subconducting States of VDAC-1

icon pap  Port-a-Patch publication in Biophysical Journal (2016)

2016 - Upregulation of Transient Receptor Potential Vanilloid Type-1 Channel Activity and Ca2+ Influx Dysfunction in Human Pterygial Cells

icon pap  Port-a-Patch publication in Investigative Opthalmology & Visual Science (2016)

2016 - The N-terminal Ankyrin Repeat Domain Is Not Required for Electrophile and Heat Activation of the Purified Mosquito TRPA1 Receptor

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Nature (2016)

2016 - Structural and functional characterization of a calcium-activated cation channel from Tsukamurella paurometabola

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Nature Communications (2016)

2016 - Reversible control of current across lipid membranes by local heating

icon pap  Port-a-Patch publication in Scientific Reports (2016)

2016 - Pore architecture of TRIC channels and insights into their gating mechanism

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Nature (2016)

2016 - Photosensitization in Porphyrias and Photodynamic Therapy Involves TRPA1 and TRPV1

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in The Journal of Neuroscience (2016)

2016 - Human TRPA1 is a heat sensor displaying intrinsic U-shaped thermosensitivity

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Scientific Reports (2016)

2016 - Functional characterization of p7 viroporin from hepatitis C virus produced in a cell-free expression system

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Protein Expression and Purification (2016)

2016 - Distinct regions that control ion selectivity and calcium-dependent activation in the bestrophin ion channel

icon pap  Port-a-Patch publication in Proceedings of the National Academy of Sciences of the United States of America (2016)

2016 - Defined Medium Conditions for the Induction and Expansion of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium

icon pap  Port-a-Patch publication in Stem Cell Reviews and Reports (2016)

2016 - Conductance and Capacity of Plain Lipid Membranes under Conditions of Variable Gravity

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Biomedical Science and Engineering (2016)

2015 - The Functional Property Changes of Muscular Nav1.4 and Cardiac Nav1.5 Induced by Scorpion Toxin BmK AGP-SYPU1 Mutants Y42F and Y5F

icon pap  Port-a-Patch publication in Biochemistry (2015)

2015 - Quantification of Fluoroquinolone Uptake through the Outer Membrane Channel OmpF of Escherichia coli

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of the American Chemical Society (2015)

2015 - K2P channel gating mechanisms revealed by structures of TREK-2 and a complex with Prozac

icon pap  Port-a-Patch publication in Science (2015)

2015 - Different Ligands of the TRPV3 Cation Channel Cause Distinct Conformational Changes As Revealed by Intrinsic Tryptophan Fluorescence Quenching

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Biological Chemistry (2015)

2015 - Cell-free expression of a functional pore-only sodium channel

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Protein Expression and Purification (2015)

2015 - Antibiotic translocation through porins studied in planar lipid bilayers using parallel platforms

Icon Orbit  Orbit 16,   icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Analyst (2015)

2014 - Ultra-stable glass microcraters for on-chip patch clamping

icon pap  Port-a-Patch publication in Joyal Society of Chemistry Advances (2014)

2014 - Studying mechanosensitive ion channels with an automated patch clamp

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in European Biophysics Journal (2014)

2014 - Structure of a Conserved Golgi Complex-targeting Signal in Coronavirus Envelope Proteins

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Biological Chemistry (2014)

2014 - Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Biosensors and Bioelectronics (2014)

2014 - L-Carnitine Reduces in Human Conjunctival Epithelial Cells Hypertonic- Induced Shrinkage through Interacting with TRPV1 Channels

icon pap  Port-a-Patch publication in Cellular Physiology and Biochemistry (2014)

2014 - KCNMA1 Encoded Cardiac BK Channels Afford Protection against Ischemia-Reperfusion Injury

icon pap  Port-a-Patch publication in PLoS One (2014)

2014 - Improvement of Chloride Transport Defect by Gonadotropin-Releasing Hormone (GnRH) in Cystic Fibrosis Epithelial Cells

icon pap  Port-a-Patch publication in PLoS One (2014)

2014 - Human TRPA1 is intrinsically cold- and chemosensitive with and without its N-terminal ankyrin repeat domain

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States of America (2014)

2014 - High susceptibility to fatty liver disease in two-pore channel 2-deficient mice

icon pap  Port-a-Patch publication in Nature Communications (2014)

2014 - Crystal structure of a human GABAA receptor

icon pap  Port-a-Patch publication in Nature (2014)

2014 - Calcium regulation by temperature-sensitive transient receptor potential channels in human uveal melanoma cells

icon pap  Port-a-Patch publication in Cellular Signalling (2014)

2014 - Automated Patch Clamp Analysis of nAChα7 and NaV1.7 Channels

icon pap  Port-a-Patch and   icon pl   Patchliner publication in Current Protocols in Pharmacology (2014)

2014 - An outer membrane channel protein of Mycobacterium tuberculosis with exotoxin activity

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States of America (2014)

2014 - A small molecule restores function to TRPML1 mutant isoforms responsible for mucolipidosis type IV

icon pap  Port-a-Patch publication in Nature Communications (2014)

2013 - Monoacylglycerols Activate TRPV1 – A Link between Phospholipase C and TRPV1

icon pap   Port-a-Patch publication in PLoS One (2013)

2013 - Functional significance of thermosensitive transient receptor potential melastatin channel 8 (TRPM8) expression in immortalized human corneal endothelial cells

icon pap  Port-a-Patch publication in Experimental Eye Research (2013)

2013 - Differential Effects of the β‐Adrenoceptor Blockers Carvedilol and Metoprolol on SQT1‐ and SQT2‐Mutant Channels

icon pap   Port-a-Patch publication in Journal of Cardiovascular Electrophysiology (2013)

2013 - Crystal structure and functional mechanism of a human antimicrobial membrane channel

icon pap   Port-a-Patch publication in Proceedings of the National Academy of Sciences of the United States of America (2013)

2012 - Thermo-sensitive transient receptor potential vanilloid channel-1 regulates intracellular calcium and triggers chromogranin A secretion in pancreatic neuroendocrine BON-1 tumor cells

icon pap   Port-a-Patch publication in Cellular Signalling (2012)

2012 - Selective modification of the N-Terminal structure of polytheonamide B significantly changes its cytotoxicity and activity as an ion channel

icon pap   Port-a-Patch publication in ChemMedChem (2012)

2012 - Regulation of Hyperpolarization-activated Cyclic Nucleotide-gated (HCN) Channel Activity by cCMP

icon pap   Port-a-Patch publication in Journal of Biological Chemistry (2012)

2012 - Natural and artificial ion channels for biosensing platforms

icon pap   Port-a-Patch,   icon pl   Patchliner,   icon sp96   SyncroPatch 96 ((a predecessor model of SyncroPatch 384PE) and   icon vpp   Vesicle Prep Pro publication in Analytical and Bioanalytical Chemistry (2012)

2012 - Isolation, characterization and total regioselective synthesis of the novel μO-conotoxin MfVIA from Conus magnificus that targets voltage-gated sodium channels

icon pap   Port-a-Patch publication in Biochemical Pharmacology (2012)

2012 - Interleukin-1R3 mediates interleukin-1–induced potassium current increase through fast activation of Akt kinase

icon pap   Port-a-Patch publication in Proceedings of the National Academy of Sciences of the Unites States of America (2012)

2012 - Insulin-secreting INS-1E cells express functional TRPV1 channels

icon pap   Port-a-Patch publication in Islets (2012)

2012 - HTS techniques for patch clamp-based ion channel screening - economy and advances

icon pap   Port-a-Patch,   icon pl   Patchliner and   icon sp96   SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) publication in Expert Opinion on Drug Discovery (2012)

2012 - Green Fluorescent Protein Changes the Conductance of Connexin 43 (Cx43) Hemichannels Reconstituted in Planar Lipid Bilayer

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in The Journal of Biological Chemistry (2012)

2012 - Effects of the Antitussive Drug Cloperastine on Ventricular Repolarization in Halothane-Anesthetized Guinea Pigs

icon pap   Port-a-Patch publication in Journal of Pharmacologigal Sciences (2012)

2012 - DNA unzipping and protein unfolding using nanopores

icon pap   Port-a-Patch publication in Methods of Molecular Biology (2012)

2012 - Design, synthesis and functional analysis of dansylated polytheonamide mimic: an artificial Peptide ion channel

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of the American Chemical Society (2012)

2012 - Calcium regulation by thermo- and osmosensing transient receptor potential vanilloid channels (TRPVs) in human conjunctival epithelial cells

icon pap   Port-a-Patch publication in Histochemistry and Cell Biology (2012)

2011 - Voltage-gated sodium channel (NaV) protein dissection creates a set of functional pore-only proteins

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States (2011)

2011 - Thermosensitive transient receptor potential channels in human corneal epithelial cells

icon pap   Port-a-Patch publication in Journal of Cellular Physiology (2011)

2011 - State-of-the-art automated patch clamp devices: heat activation, action potentials, and high throughput in ion channel screening

icon pap   Port-a-Patch,   icon pl  Patchliner and   icon sp96   SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) publication in Frontiers in Pharmacology (2011)

2011 - Physiology of the Human Corneal Endothelium – New Insights from Electrophysiological Investigations

icon pap   Port-a-Patch publication in Klinische Monatsblätter für Augenheilkunde (2011)

2011 - Functional Analysis of Synthetic Substructures of Polytheonamide B: A Transmembrane Channel-Forming Peptide

icon pap   Port-a-Patch publication in Angewandte Chemie International Edition (2011)

2011 - Chemical and biological characterization of four new linear cationic a-helical peptides from the venoms of two solitary eumenine wasps

icon pap   Port-a-Patch publication in Toxicon (2011)

2011 - Characterization of transient receptor potential vanilloid channel 4 (TRPV4) in human corneal endothelial cells

icon pap   Port-a-Patch publication in Experimental Eye Research (2011)

2011 - Cardiac Toxicity

icon pl   Patchliner and   icon pap   Port-a-Patch book chapter in ADMET for Medicinal Chemists: A Practical Guide (2011)

2011 - Automated Patch Clamp on mESC-Derived Cardiomyocytes for Cardiotoxicity Prediction

icon pl   Patchliner and   icon pap   Port-a-Patch publication in Journal of Biological Chemistry (2011)

2010 - TRPV channels mediate temperature-sensing in human corneal endothelial cells

icon pap  Port-a-Patch publication in Experimental Eye Research (2010)

2010 - Studying mechanosensitive ion channels using liposomes

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Methods in Molecular Biology (2010)

2010 - Renaissance of ion channel research and drug discovery by patch clamp automation

icon pap  Port-a-Patch,   icon pl   Patchliner and   icon sp96   SyncroPatch 96 (a predecessor model of SyncroPatch 384PE)  publication in Future Medical Chemistry (2010)

2010 - Planar Patch Clamp Approach to Characterize Ionic Currents from Intact Lysosomes

icon pap  Port-a-Patch publication in Science Signaling (2010)

2010 - Permeation of antibiotics through escherichia coli OmpF and OmpC porins: screening for influx on a single-molecule level

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Biomolecular Screening (2010)

2010 - Cor.At Cardiomyocytes: Primary-like Cardiomyocytes for Manual and Automated Electrophysiological Screening

icon pap  Port-a-Patch and   icon pl   Patchliner publication in Lonza Resource Notes (2010)

2010 - Characterization of two pore channel 2 (TPCN2) -mediated Ca2+ currents in isolated lysosomes

icon pap  Port-a-Patch publication in The Journal of Biological Chemistry (2010)

2009 - Transient receptor potential ankyrin 1 antagonists block the noxious effects of toxic industrial isocyanates and tear gases

icon pap  Port-a-Patch publication in FASEB (2009)

2009 - The M34A mutant of Connexin26 reveals active conductance states in pore-suspending membranes

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Structural Biology (2009)

2009 - T-type channel blocking properties and antiabsence activity of two imidazo[1,2-b]pyridazine derivatives structurally related to indomethacin

icon pap  Port-a-Patch publication in Neuropharmacology (2009)

2009 - Port-a-Patch and Patchliner: High fidelity electrophysiology for secondary screening and safety pharmacology

icon pap  Port-a-Patch and   icon pl   Patchliner publication in Combinatorial Chemistry & High Throughput Screening (2009)

2009 - Amyloid-beta-Induced Ion Flux in Artificial Lipid Bilayers and Neuronal Cells: Resolving a Controversy

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Neurotoxicity Research (2009)

2008 - TRPA1 is a major oxidant sensor in murine airway sensory neurons

icon pap  Port-a-Patch publication in The Journal of Clinical Investigation (2008)

2008 - Synthesis and biological evaluation of chalcones as inhibitors of the voltage-gated potassium channel Kv1.3

icon pap  Port-a-Patch publication in Bioorganic & Medicinal Chemistry Letters (2008)

2008 - Rapid screening of membrane protein activity: electrophysiological analysis of OmpF reconstituted in proteoliposomes

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Lab-on-a-chip (2008)

2008 - Planar patch-clamp force microscopy on living cells

icon pap  Port-a-Patch publication in Ultramicroscopy (2008)

2008 - Planar patch clamp: Advances in electrophysiology

icon pap  Port-a-Patch book chapter in "Potassium Channels" (2008)

2008 - Ion channel screening – automated patch clamp on the rise

icon pap  Port-a-Patch and   icon pl   Patchliner publication in Drug Discovery Today (2008)

2008 - High-throughput electrophysiology: an emerging paradigm for ion-channel screening and physiology

icon pap  Port-a-Patch publication in Nature Reviews Drug Discovery (2008)

2007 - Planar Patch Clamping

icon pap  Port-a-Patch and   icon pl   Patchliner book chapter in "Patch Clamp Analysis – Advanced Techniques", Series: Neuromethods (2007)

2007 - Electrophysiological assessment of HERG blockade: Comparative study using automated and conventional patch clamp systems

icon pap  Port-a-Patch publication in Toxicological Letters (2007)

2007 - Automated ion channel screening: patch clamping made easy

icon pap  Port-a-Patch and   icon pl   Patchliner publication in Expert Opinion Therapeutic Targets (2007)

2006 - Microchip technology for automated and parallel patch clamp recording

icon pap  Port-a-Patch and   icon pl   Patchliner publication in Small Journal (2006)

2006 - High-resolution electrophysiology on a chip: Transient dynamics of alamethicin channel formation

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Biochimica et Biophysica Acta - Biomembranes (2006)

2005 - The Port-a-Patch: The smallest patch clamp set up for high quality electrophysiology

icon pap  Port-a-Patch publication in HEKA Impulse (2005)

2005 - Cytotoxicity of colloidal CdSe and CdSe/ZnS nanoparticles

icon pap  Port-a-Patch publication in Nano Letters (2005)

2004 - Ion channel drug discovery and research: The automated Nano-Patch-Clamp technology

icon pap  Port-a-Patch publication in Current Drug Discovery Technologies (2004)

2003 - Simultaneous optical and electrical recording of single gramicidin channels

icon pap  Port-a-Patch publication in Biophysical Journal (2003)

2003 - Microstructured apertures in planar glass substrates for ion channel research

icon pap  Port-a-Patch publication in Channels and Receptors (2003)

2003 - Lighting up single ion channels

icon pap  Port-a-Patch publication in Biophysical Journal (2003)

2003 - High quality ion channel analysis on a chip with the NPC-technology

icon pap  Port-a-Patch publication in Assay and Drug Development Technologies (2003)

2002 - Whole cell patch clamp recording performed on a planar glass chip

icon pap  Port-a-Patch publication in Biophysical Journal (2002)

2002 - Patch clamp on a chip

icon pap  Port-a-Patch publication in Biophysical Journal (2002)

2002 - Activity of single ion channel proteins detected with a planar microstructure

icon pap  Port-a-Patch publication in Applied Physics Letters (2002)

2001 - Microstructured glass chip for ion channel electrophysiology

icon pap  Port-a-Patch publication in Physical review. E, Statistical, nonlinear, and soft matter physics (2001)

2000 - Stable integration of isolated cell membrane patches in a nanomachined aperture

icon pap  Port-a-Patch publication in Applied Physics Letters (2000)

Posters

2015 - "Organellar Transporters and Ion Channels - How to access their electrophysiology by using the SURFE2R technology and Planar Patch Clamp"

Icon N1   SURFE²R N1 and   icon sp96   SyncroPatch 96 (a predecessor model of the SyncroPatch 384PE) and   icon pap   Port-a-Patch poster, GRC - Organellar Channels and Transporters 2015   logo pdf   (1.6 MB)

2015 - "The backstage pass to study your favorite TRP channel"

icon pap   Port-a-Patch and   icon pl   Patchliner and SyncroPatch 384PE and   icon sp96   SyncroPatch 384PE poster, TRP Meeting 2015   logo pdf   (2.2 MB)

Contact Us

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Nanion Technologies GmbH

Ganghoferstr. 70A
D-80339 Munich - Germany
info@nanion.de