• SURFE2R 96SE

    Fully automated data recording and analysis. 10,000 data points per day!
  • SURFE2R 96SE

    First high throughput instrument on the market for SSM-based electrophysiology
  • SURFE2R 96SE

    Finally high throughput label-free functional assays for transporters available
  • SURFE2R 96SE

    High signal amplification compared to patch-clamp: transport & binding assays
  • SURFE2R 96SE

    Turn-key system for efficient transporter protein analysis

2013 - Measuring Interference of Drug-Like Molecules with the Respiratory Chain: Toward the Early Identification of Mitochondrial Uncouplers in Lead Finding

Icon 96SE   SURFE²R N96 (predesessor model of SURFE²R 96SE) publication in Assay and Drug Development Technologies (2013)

Authors: 
Stock U., Matter H., Diekert K., Dörner W., Dröse S., Licher T.

Journal: 
Assay Drug Dev Technol. (2013) 11(7):408–422


Abstract: 

The electron transport chain (ETC) couples electron transfer between donors and acceptors with proton transport across the inner mitochondrial membrane. The resulting electrochemical proton gradient is used to generate chemical energy in the form of adenosine triphosphate (ATP). Proton transfer is based on the activity of complex I–V proteins in the ETC. The overall electrical activity of these proteins can be measured by proton transfer using Solid Supported Membrane technology. We tested the activity of complexes I, III, and V in a combined assay, called oxidative phosphorylation assay (oxphos assay), by activating each complex with the corresponding substrate. The oxphos assay was used to test in-house substances from different projects and several drugs currently available on the market that have reported effects on mitochondrial functions. The resulting data were compared to the influence of the respective compounds on mitochondria as determined by oxygen consumption and to data generated with an ATP depletion assay. The comparison shows that the oxidative phosphorylation assay provides both a rapid approach for detecting interaction of compounds with respiratory chain proteins and information on their mode of interaction. Therefore, the oxphos assay is a useful tool to support structure activity relationship studies by allowing early identification of mitotoxicity and for analyzing the outcome of phenotypic screens that are susceptible to the generation of mitotoxicity-related artifacts.


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