Nanion Technologies
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Data and Applications


Bilayer Recordings on the Patchliner


p37_2_BilayRec

With suction the GUVs are attracted to the aperture. As soon as one GUV hits the glass substrate, it bursts and forms a bilayer across the aperture. Shown are single channel recordings from gramicidin which was incorprated into the bilayer after its formation. Traces were recorded in 100 mM HCl at −100 mV.

Bilayer Recordings of KcsA


KCSA-Vesicle-prep-pro

The image shows a representative current trace recorded from a planar lipid bilayer in which purified KcsA channels were reconstituted (100 mM KCl, asymmetric pH; pH 4/pH 7). A ramp from -100 mV to +100 mV was used to evoke the single channel events.

Download: Application Note

Bilayer Recordings of OmpF


OMPFPolyamine_bea

Channels were activated in the presence of spermine (0.5 mM). The downward spikes represent the closure events due to the effect of spermine.

Data were kindly supplied by Prof. Mathias Winterhalter, Jacobs University, Bremen, Germany.

DNA Translocation in Hemolysin


p45_4_AlphaHemolysinNeu

Reconstituted Alpha hemolysin channels are constantly open at positive and negative membrane potentials. Gating is observed as a result of the passage of a single stranded DNA molecule through the pore. Recordings were performed on the Port-a-Patch®.

Data were kindly supplied by Prof. Fritz Simmel, Technical University of Munich, Munich, Germany.

GUVs

p44_1_GUV

The GUVs produced with the Vesicle Prep Pro® typically range from 1 – 30 μm in diameter, with an average diameter of
approximately 10 μm (60-70%).

The Image was kindly provided of Dr. Sonderman/Prof. Behrends, University of Freiburg, Germany.

Reconstitution of IP3 Receptors


IP3_bea_vereinfacht

Representative traces of the IP3 (inositol-triphosphate) receptor were recorded at different voltages in the presence of IP3 (0.2 mM free Ca2+ and 1 mM Na2ATP, 140 mM KCl). Addition of 2 μM IP3 on the cytosolic side evoked openings of the IP3 receptor.

Data were kindly supplied by Prof. Colin Taylor, University of Cambridge, Cambridge, UK.

TRPM8


TrpM8_420x420

Shown are single channel events of TRPM8 reconstituted in a planar lipid bilayer. The recordings were made with the with the Port-a-Patch®. The channel was activated by PIP2 and methanol. Currents were recorded at 100 mV.

Data kindly provided by Dr. Zakharian and Dr. Rohac From UMDNJ New Jersey Medical School, Newark, NJ 07103, USA